Here is a "best of" collection of links to manuals, tools, and other resources for Agilent Cell Analysis products. For other instruments refer to Collection of Support Resources.
Collection of Cell Analysis Resources
See also the new Cell Analysis FAQs and Troubleshooting
Seahorse
Overall:
Cell Analysis FAQs and Troubleshooting
Agilent Seahorse XF Media Selection Guide
Seahorse XF Assay Learning Center
Publications with Agilent Cell Analysis Data
Knowing your Cells of Interest: Advice and Suggestions for a Successful XF Experience
Agilent Seahorse Software Specifications
XFp
Instrument User Guide
XFp Extracellular Flux Analyzer User Guide
XFp Kits – User Guides
Agilent Seahorse XFp Real-Time ATP Rate Assay Kit
Agilent Seahorse XFp Cell Mito Stress Test Kit
Agilent Seahorse XFp Glycolytic Rate Assay Kit
Agilent Seahorse XFp Mito Fuel Flex Test Kit
Agilent Seahorse XF Palmitate-BSA FAO Substrate Guide
Agilent Seahorse XF Plasma Membrane Permeabilizer (PMP) Guide
Agilent Seahorse XFp Glycolysis Stress Test Kit
Site Preparation Checklist
XFp Site Preparation Checklist
Overall User Guides
Cell Characterization: The XFp Analyzer and the XF Real-Time ATP Rate Assay
Seeding Adherent Cells in Agilent Seahorse XFp Cell Culture Miniplates
How to Hydrate an Agilent Seahorse XFp Sensor Cartridge
Washing Adherent Cells in Agilent Seahorse XFp Cell Culture Miniplates
Seeding Suspension Cells in Agilent Seahorse XFp Cell Culture Miniplates
Loading the Agilent Seahorse XFp Sensor Cartridge Injection Ports
XF/XFe
XFe Instrument User Guide
XFe Extracellular Flux Analyzer User Guide (Version 2.6)
XFe Kits – User Guides
Agilent Seahorse XF Real-Time ATP Rate Assay Kit
Agilent Seahorse XF Palmitate-BSA FAO Substrate Quickstart Guide
Agilent Seahorse XF Cell Mito Stress Test Kit
Agilent Seahorse XF Glycolytic Rate Assay Kit
Agilent Seahorse XF Mito Fuel Flex Test Kit
Agilent Seahorse XF Palmitate-BSA FAO Substrate Guide
Agilent Seahorse XF Plasma Membrane Permeabilizer (PMP) Guide
Agilent Seahorse XF Glycolysis Stress Test Kit
Site Preparation Checklists
XFe96 Site Preparation Checklist
XFe24 Site Preparation Checklist
Normalization – User Guides
Seahorse XF Imaging and Normalization System XFe24 Hoechst33342 Staining Optimization Guidelines
Seahorse XF Imaging and Normalization System XFe96 Hoechst33342 Staining Optimization Guidelines
Hydration – User Guides
How to Hydrate an Agilent Seahorse XFe96 Sensor Cartridge
Loading Injection Ports – User Guides
Loading the Agilent Seahorse XF24 Sensor Cartridge Injection Ports
Loading the Agilent Seahorse XFe96 Sensor Cartridge Injection Ports
Loading the Agilent Seahorse XFe24 Sensor Cartridge Injection Ports
Informative and How-To Videos
Bravo Automated Workflow for Seahorse XFe96 Sample Preparation
How to analyze Agilent XF Real-Time ATP Rate Assay result data
Agilent Seahorse XF Analyzer Multi-port Drug Injection Video
Measuring Cell Metabolism - Transient Micro-chamber Video
Wave
Agilent Seahorse XFe96 and XFe24 Analyzers (For use with Wave Desktop 2.4) User Guide
Wave 2.6 User Guide For use with Wave Desktop software and Agilent Seahorse XFe Analyzers
Running Wave Desktop 2.6 on a Mac
Download Hypoxia License Installer
Articles and Whitepapers:
Improving Quantification of Cellular Glycolytic Rate Using Agilent Seahorse XF Technology (Link to download, not straight to paper)
Quantifying Cellular ATP Production Rate Using Agilent Seahorse XF Technology
Technical Overviews
Modifying Assay Guides for use with Agilent Seahorse XFe24 and XF24 Analyzers
Islet Assay Using the Agilent Seahorse XF24 Islet Capture Microplate
Using PMP to Measure Mitochondrial ETC Complex Activity in Limited Biomaterials
3T3-L1 Pre-Adipocyte Differentiation
Methods and strategies for normalizing XF metabolic data to cellular parameters
Conducting an XFe Assay in an Hypoxia Chamber at ≥3% O² *
Using PMP to Measure Substrate Specific ETC/OxPhos Activity in Permeabilized Cells
Application Notes
Application Notes (overall link)
Assessing functional metabolism using cells sourced from blood
Assessing mitochondrial dysfunction in primary cardiomyocytes
Assessing the impact of autophagy on cellular metabolism
Assessing therapies for traumatic brain or spinal cord injuries
Bioenergetic analysis of primary neuronal deficiencies
Bioenergetic analysis of suspension cells: hematopoietic stem cells and lymphocytes
Defining metabolic phenotypes in cancer cells
Determining Metabolic Switch in Induced Pluripotent Stem Cells (iPSCs)
Direct Measurements of Cellular Metabolism for Identification of Mitochondrial Drug Targets
Evaluating changes in cell metabolism in neuroimmune and neuropsychiatric disorders
Functional validation of Genomic and Proteomic data
Measuring bioenergetic profiles of human adipocytes
Measuring functional metabolism of brite adipocytes
Measuring glycolysis and oxidative metabolism in cancer cells
Measuring glycolytic function in cells
Measuring the metabolic switch in cancer cells
Metabolic Pathway Determination Using the Seahorse XF24 Analyzer
Normalization of Agilent Seahorse XF Data by In-situ Cell Counting Using a BioTek Cytation 5
Quantitative Analysis of Neutrophil Activation Using Agilent Seahorse XF Technology
Rapid, Real-time Detection of T Cell Activation Using an Agilent Seahorse XF Analyzer
Real Time Discrimination of Inflammatory Macrophage Activation Using Agilent Seahorse XF Technology
XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System
Quick Reference Guides
Quick Reference Guide: Calculating Proton Efflux Rate (PER) Data
Agilent Seahorse XF Buffer Factor Protocol Quick Reference Guide
Videos
Webinars
Webinars (overall link)
Abnormal Respiratory Function in Autism Immune Cells
Adaptation of Microplate-based Respirometry for Hippocampal Brain Slices
An MTCH2 pathway repressing mitochondria metabolism regulates haematopoietic stem cell fate
Analysis of Glutaminolysis and Fumarate Respiration in Bcl-xL-expressing Cells
Assessing bioenergetic function in response to oxidative stress by metabolic profiling
Assaying Isolated Mitochondria in the Seahorse XF24: Learning More while Requiring Less
Biochemical analysis in C. elegans as a model for metabolic disease and aging
Bioenergetic Evaluation in Mitochondrial Disorders
Characterization of Potential Bioenergetic Medicine Agents
Combining Seahorse XF Analysis with Stable Isotope Tracing to Reveal Novel Metabolic Drug Targets
Defining the Critical Bioenergetic Function of Huntingtin Protein
De-risk Your Drug Discovery Pipeline
Exploring how Mycobacterium tuberculosis reprograms host bioenergetic metabolism
Exploring the metabolism of CAR-T cells
Fueling T cells in Immunity and Cancer
Human Disorders of Bioenergetics
iPSC-derived neural cells for drug discovery of mitochondrial disorders
Measuring ATP production rates with the Seahorse XF Analyzer
Measuring Functional Metabolism of Tissues in 3D
Measuring platelet bioenergetic function - from marker to mechanism of disease
Metabolic Adaptations in Fumarate Hydratase Deficient Cells
Metabolic Plasticity as a Therapeutic Target
Metabolic plasticity in T cell responses: Implications for adaptive immunity
Metabolic reprogramming as a therapeutic strategy for cutaneous squamous cell carcinoma
Metabolic reprogramming in macrophages during inflammation
Metabolic Reprogramming Therapy in the Treatment of Cancer
Metabolic switches that drive stem cell specification and differentiation to a mature neuron
Metabolomics - Following the Carbons that Fuel Cancer
Mitochondria - the Powerhouse of the Cell Goes from Bench to Bedside
Mitochondrial Bioenergetics in Diabetic Cardiomyopathy
Mitochondrial Control in Health & Disease
Mitochondrial pyruvate metabolism controls neuronal function and excitotoxic death
Mitochondrial Profiling in Cells Under Physiological Conditions and In Response to Stress
Multidimensional control of mitochondrial energy production: learning from physiology and pathology
New solutions for measuring metabolism; harnessing the power of your plate reader
Normalizing Your Seahorse XF Data with Automated Cell Counting
Probe the Metabolic Depths of Tumor Spheroids Using the XFe96 Analyzer
Programming the Mitochondrial Choice of Fuel Substrates: Metabolic Control of Neuronal Excitability
Regulation of cancer cell growth by metabolic substrates
Regulating oxygen metabolism can radiosensitize solid tumors
Quantifying macrophage activation using Agilent Seahorse XF technology
Quantitative analysis of neutrophil activation using Agilent Seahorse XF technology
Seahorse Metabolic Analyzers Enable Rapid, Quantitative Measurement of Cellular Bioenergetics
Simultaneously Measuring Oxidation of Exogenous and Endogenous Fatty Acids
Systems-based approach to characterizing human pluripotent stem cell metabolism
T cell metabolism in activation and exhaustion
The Hitchhiker's Guide to XF Bioenergetic Assays
The Rate and Coupling Efficiency of Oxidative Phosphorylation in Mitochondria and Cells
Type 1 Interferons Induce Changes in Core Metabolism that Are Critical for Immune Function
Tutorial: Measuring Bioenergetics in a Variety of Cell Types
Tutorial 2: Measuring Bioenergetics in Primary Cell Types
Using a novel permeabilization reagent to uncover mechanisms of mitochondrial dysfunction
Using Reserve Capacity to Screen for Mitochondrial Toxicities and Biogenesis
Using the XF Analyzer to measure cellular respiration and glycolysis under hypoxic conditions
What's Fueling Mitochondria? Designing and Interpreting Pathway-Specific XF Assays
Working Towards Defining a Metabolic Roadmap for Stem Cell Lineage Progression
XF Glycolysis Stress Test Assesses Glycolysis and Glycolytic Capacity in Cells
Report Generators – Single and Multi-file (if available)
Seahorse XF Cell Mito Stress Test Report Generators
Seahorse XF Glycolytic Rate Assay Report Generators
Seahorse XF Mito Fuel Flex Test Report Generator
Seahorse XF Real-Time ATP Rate Assay Report Generators
Soluble Sensors
Overall:
MitoXpress Xtra Oxygen Consumption Assay Plate Reader compatibility chart
Ph-Xtra Glycolysis Assay Plate Reader compatibility chart
MitoXpress Intra Plate Reader Compatibility Guide
Instrument Setup Guides – MitoXpress Xtra Oxygen Consumption Assay Setup Guides
MitoXpress Xtra Oxygen Consumption Assay Instrument setup guide for Perkin Elmer plate readers
MitoXpress Xtra Oxygen Consumption Assay Instrument setup guide for Tecan plate readers
MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for BMG Plate Readers
MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for Molecular Devices Plate Readers
MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for BioTek Plate Readers
Instrument Setup Guides – pH-Xtra Glycolysis Assay Setup Guides
pH-Xtra Glycolysis Assay Instrument setup guide for Perkin Elmer plate readers
pH-Xtra Glycolysis Assay Instrument setup guide for Tecan plate readers
pH-Xtra Glycolysis Assay Instrument Set Up Guide for BioTek Plate Readers
pH-Xtra Glycolysis Assay Instrument Set Up Guide for Molecular Devices Plate Readers
pH-Xtra Glycolysis Assay Instrument Set Up Guide for BMG Plate Readers
User Manuals
Agilent pH-Xtra Glycolysis Assay
Agilent MitoXpress Xtra Oxygen Consumption Assay (HS Method)
Agilent MitoXpress Intra Intracellular Oxygen Assay
Application Notes
Screening for Drug-Induced Mitochondrial Dysfunction Using MitoXpress Xtra on Isolated Mitochondria
Software
Agilent MitoXpress & pH-Xtra Data Visualization Tool User Manual
Webinars
New solutions for measuring metabolism; harnessing the power of your plate reader
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