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All recent questions and discussions
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Answered
What scale factor value I must use to normalize data using protein concentration?
+1
305
views
3
replies
Latest
1 month ago
by
Courtney Watts
Answered
DMEM, low glucose, pyruvate, no glutamine, no phenol red
+1
261
views
1
reply
Latest
1 month ago
by
Courtney Watts
Answered
How to measure Mitochondrial acidification from Glycolytic Rate Assay?
+1
241
views
1
reply
Latest
2 months ago
by
Courtney Watts
Answered
Oligomycin is not working
+1
443
views
4
replies
Latest
2 months ago
by
Chanyoung
Answered
C2C12 Cell detachment from 96 well plate
+1
472
views
5
replies
Latest
3 months ago
by
Courtney Watts
Answered
Barcode reader offline for Seahorse XFe96
0
1981
views
1
reply
Latest
3 months ago
by
Courtney Watts
Answered
Low BASAL OCR
+1
608
views
1
reply
Latest
3 months ago
by
Courtney Watts
Answered
non-mitochondrial consumption value for Ctrl vehicle is 6.20 to -23.6
+1
1131
views
3
replies
Latest
3 months ago
by
Courtney Watts
Answered
Oligo/FCCP/Rot/AA drug recommended conc for human melanoma cell lines (A375 and SKMEL5)
+1
923
views
2
replies
Latest
3 months ago
by
Courtney Watts
Answered
Inter-Plate Variation
+1
703
views
3
replies
Latest
3 months ago
by
Courtney Watts
Answered
No response to oligomycin during glycolysis stress test with intestinal organoids
+1
625
views
3
replies
Latest
3 months ago
by
Courtney Watts
Answered
Agilent Seahorse XF RPMI Medium
+1
666
views
5
replies
Latest
3 months ago
by
Courtney Watts
Answered
Low OCR values for ATP rate assay kit on IPEC-J2 cells
+1
1224
views
2
replies
Latest
3 months ago
by
Courtney Watts
Answered
Is it acceptable to substitute EDTA for EGTA when preparing the Mitochondrial Assay Solution (MAS, 1X)？
+1
459
views
1
reply
Latest
4 months ago
by
Courtney Watts
Answered
Seahorse on isolated mitochondria
+1
1512
views
2
replies
Latest
4 months ago
by
Gaurav Verma
Answered
What solvent for respiration reagent on isolated mitochondria assays?
+1
293
views
1
reply
Latest
5 months ago
by
Courtney Watts
Answered
Is there have Finished kit for Coupling or Electron Flow measurement in isolated mitochondrial with XFe24 or XFe96?
+1
392
views
3
replies
Latest
5 months ago
by
Courtney Watts
Answered
OD measurement in BioTek Epoch 2 Microplate Spectrophotometer
+1
479
views
1
reply
Latest
5 months ago
by
Courtney Watts
Answered
Extracellular Flux Cartridge
+1
925
views
5
replies
Latest
6 months ago
by
Courtney Watts
Answered
C02 value for ATP calculations
+1
1350
views
8
replies
Latest
6 months ago
by
Courtney Watts
Answered
Drug injection fail
+1
6133
views
10
replies
Latest
7 months ago
by
Courtney Watts
Answered
Non-glycolytic acidification: last measure before glucose, or lowest ECAR after 2-DG?
+2
5465
views
9
replies
Latest
8 months ago
by
Courtney Watts
Answered
Primary human skeletal muscle cells
+1
688
views
1
reply
Latest
8 months ago
by
Courtney Watts
Answered
What combination of substrate I need to use on my isolated mitochondria on seahorse plate to keep them respiring?
+1
467
views
3
replies
Latest
8 months ago
by
Courtney Watts
Answered
Seahorse XF Palmitate Oxidation Stress Test in B16F10 cells
+1
958
views
2
replies
Latest
11 months ago
by
yadachi
Answered
How to know and detect signal not use the RTCA?
+1
934
views
1
reply
Latest
over 1 year ago
by
Courtney Watts
Answered
Barcode errors - help?
+1
1472
views
1
reply
Latest
over 1 year ago
by
Courtney Watts
Answered
Seahorse plate orientation and data analysis
+1
1475
views
2
replies
Latest
over 1 year ago
by
TEve
Answered
Mito Fuel Flex Test
+1
1107
views
1
reply
Latest
over 1 year ago
by
Courtney Watts
Answered
Cell metrics export
+1
2525
views
2
replies
Latest
over 1 year ago
by
WJCannan
Answered
How to export Bam files from Agilent Surecall software
+1
3018
views
2
replies
Latest
over 1 year ago
by
Tom1884it
Answered
plate coating
+1
2977
views
3
replies
Latest
over 1 year ago
by
Courtney Watts
Answered
Glycolytic rate assay with PBMCs
+1
1861
views
1
reply
Latest
over 1 year ago
by
Courtney Watts
Answered
Different kits at the same time
+1
2161
views
1
reply
Latest
over 2 years ago
by
Courtney Watts
Answered
getting most of ECAR data from a mito-stress test
+1
5960
views
1
reply
Latest
over 2 years ago
by
Courtney Watts
Answered
Seahorse Xfe96 mito stress maximum OCR
+1
3637
views
3
replies
Latest
over 2 years ago
by
Courtney Watts
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