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All recent questions and discussions
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Answered
BSA quantification using normalize function for a cell mito stress test
+1
131
views
1
reply
Latest
1 month ago
by
Courtney Watts
Answered
Shelf life of XFe96 reagents
+1
132
views
1
reply
Latest
1 month ago
by
Courtney Watts
Answered
Using Mito Stress and Glycolysis Stress Test in the same 96 well plate
+1
267
views
1
reply
Latest
1 month ago
by
Courtney Watts
Answered
When I use the Agilent Seahorse XF Mito Fuel Flex Test Kit to detect the flexibility, why the values are significant negative, both of them are lower than -5%.
+1
437
views
1
reply
Latest
3 months ago
by
Courtney Watts
Answered
Scale Factor Value for Normalization of Seahorse Data When Using Protein Concentration
+1
1114
views
3
replies
Latest
6 months ago
by
Courtney Watts
Answered
DMEM, low glucose, pyruvate, no glutamine, no phenol red
+1
943
views
1
reply
Latest
6 months ago
by
Courtney Watts
Answered
How to measure Mitochondrial acidification from Glycolytic Rate Assay?
+1
818
views
1
reply
Latest
6 months ago
by
Courtney Watts
Answered
Oligomycin is not working
+1
1196
views
4
replies
Latest
7 months ago
by
Chanyoung
Answered
C2C12 Cell detachment from 96 well plate
+1
1463
views
5
replies
Latest
7 months ago
by
Courtney Watts
Answered
Barcode reader offline for Seahorse XFe96
0
2622
views
1
reply
Latest
7 months ago
by
Courtney Watts
Answered
Low BASAL OCR
+1
1089
views
1
reply
Latest
8 months ago
by
Courtney Watts
Answered
non-mitochondrial consumption value for Ctrl vehicle is 6.20 to -23.6
+1
1754
views
3
replies
Latest
8 months ago
by
Courtney Watts
Answered
Oligo/FCCP/Rot/AA drug recommended conc for human melanoma cell lines (A375 and SKMEL5)
+1
1563
views
2
replies
Latest
8 months ago
by
Courtney Watts
Answered
Inter-Plate Variation
+1
1405
views
3
replies
Latest
8 months ago
by
Courtney Watts
Answered
No response to oligomycin during glycolysis stress test with intestinal organoids
+1
1231
views
3
replies
Latest
8 months ago
by
Courtney Watts
Answered
Agilent Seahorse XF RPMI Medium
+1
1405
views
5
replies
Latest
8 months ago
by
Courtney Watts
Answered
Low OCR values for ATP rate assay kit on IPEC-J2 cells
+1
1843
views
2
replies
Latest
8 months ago
by
Courtney Watts
Answered
Is it acceptable to substitute EDTA for EGTA when preparing the Mitochondrial Assay Solution (MAS, 1X)？
+1
1099
views
1
reply
Latest
8 months ago
by
Courtney Watts
Answered
Seahorse on isolated mitochondria
+1
2351
views
2
replies
Latest
8 months ago
by
Gaurav Verma
Answered
What solvent for respiration reagent on isolated mitochondria assays?
+1
770
views
1
reply
Latest
9 months ago
by
Courtney Watts
Answered
Is there have Finished kit for Coupling or Electron Flow measurement in isolated mitochondrial with XFe24 or XFe96?
+1
963
views
3
replies
Latest
9 months ago
by
Courtney Watts
Answered
OD measurement in BioTek Epoch 2 Microplate Spectrophotometer
+1
974
views
1
reply
Latest
10 months ago
by
Courtney Watts
Answered
Extracellular Flux Cartridge
+1
1680
views
5
replies
Latest
10 months ago
by
Courtney Watts
Answered
C02 value for ATP calculations
+1
2262
views
8
replies
Latest
11 months ago
by
Courtney Watts
Answered
Drug injection fail
+1
7293
views
10
replies
Latest
11 months ago
by
Courtney Watts
Answered
Non-glycolytic acidification: last measure before glucose, or lowest ECAR after 2-DG?
+2
6634
views
9
replies
Latest
over 1 year ago
by
Courtney Watts
Answered
Primary human skeletal muscle cells
+1
1334
views
1
reply
Latest
over 1 year ago
by
Courtney Watts
Answered
What combination of substrate I need to use on my isolated mitochondria on seahorse plate to keep them respiring?
+1
1133
views
3
replies
Latest
over 1 year ago
by
Courtney Watts
Answered
Seahorse XF Palmitate Oxidation Stress Test in B16F10 cells
+1
1522
views
2
replies
Latest
over 1 year ago
by
yadachi
Answered
How to know and detect signal not use the RTCA?
+1
1443
views
1
reply
Latest
over 1 year ago
by
Courtney Watts
Answered
Seahorse XFe96 Barcode Read Errors
+1
2224
views
1
reply
Latest
over 1 year ago
by
Courtney Watts
Answered
Seahorse plate orientation and data analysis
+1
2043
views
2
replies
Latest
over 1 year ago
by
TEve
Answered
Mito Fuel Flex Test
+1
1557
views
1
reply
Latest
over 1 year ago
by
Courtney Watts
Answered
Cell metrics export
+1
3469
views
2
replies
Latest
over 1 year ago
by
WJCannan
Answered
How to export Bam files from Agilent Surecall software
+1
3551
views
2
replies
Latest
over 2 years ago
by
Tom1884it
Answered
plate coating
+1
3799
views
3
replies
Latest
over 2 years ago
by
Courtney Watts
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