What do I do if I cannot detect any signal in wells containing cells and MitoXpress Intra (or I can detect a signal but the slope (rate) appears very low)?
Check correct Instrument Settings (“Appendix A - Instrument Settings” on page 27)- Perform Signal Optimization - Include Signal controls as described previously - Increase cell density. If tested and not resolved, contact www.agilent.com.
What do I do if I can detect a signal in wells containing MitoXpress Intra loaded cells, but the signal level falls initially or is variable from well to well?
Check cell seeding and pipetting consistency; increase cell density; ensure plate, instrument; and all culture media and stock solutions are prewarmed at 37 °C before use; reduce plate preparation times.
More FAQs: Cell Analysis FAQs and Troubleshooting
Keywords: MitoXPress Intra, MitoXpress Intra Oxygen Assay, Signal, Slope, Soluble Sensors, FAQ