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Courtney WattsHere is a "best of" collection of links to manuals, tools, and other resources for Agilent Cell Analysis products. For other instruments refer to Collection of Support Resources.
Collection of Cell Analysis Resources
See also the new Cell Analysis FAQs and Troubleshooting
Seahorse
Overall:
Cell Analysis FAQs and Troubleshooting
Agilent Seahorse XF Media Selection Guide
Seahorse XF Assay Learning Center
Publications with Agilent Cell Analysis Data
Knowing your Cells of Interest: Advice and Suggestions for a Successful XF Experience
Characterizing Your Cells Using OCR Values to Determine Optimal Seeding Density
Agilent Seahorse Software Specifications
XFp and/or HS Mini
Instrument User Guides
XFp Extracellular Flux Analyzer User Guide
Seahorse XF HS Mini Extracellular Flux Analyzer User Manual
XFp and HS Mini Kits – User Guides
Agilent Seahorse XFp Real-Time ATP Rate Assay Kit
Agilent Seahorse XFp Cell Mito Stress Test Kit
Agilent Seahorse XFp Glycolytic Rate Assay Kit
Agilent Seahorse XFp Mito Fuel Flex Test Kit
Agilent Seahorse XF Palmitate-BSA FAO Substrate Guide
Agilent Seahorse XF Plasma Membrane Permeabilizer (PMP) Guide
Agilent Seahorse XFp Glycolysis Stress Test Kit
Agilent Seahorse XFp T Cell Metabolic Profiling Kit
Site Preparation Checklist
XFp Site Preparation Checklist
HS Mini Site Preparation Checklist
Overall User Guides
XFp:
Cell Characterization: The XFp Analyzer and the XF Real-Time ATP Rate Assay
Seeding Adherent Cells in Agilent Seahorse XFp Cell Culture Miniplates
How to Hydrate an Agilent Seahorse XFp Sensor Cartridge
Washing Adherent Cells in Agilent Seahorse XFp Cell Culture Miniplates
Seeding Suspension Cells in Agilent Seahorse XFp Cell Culture Miniplates
Loading the Agilent Seahorse XFp Sensor Cartridge Injection Ports
HS Mini:
Cell Characterization: The XF HS Mini Analyzer and the XF Real-Time ATP Rate Assay
Seeding Adherent Cells in Agilent Seahorse XF HS Miniplates
Seeding Suspension Cells in Agilent Seahorse XF HS PDL Miniplates
How to Hydrate an Agilent Seahorse XFp Sensor Cartridge
Loading the Agilent Seahorse XFp Sensor Cartridge Injection Ports
XF/XFe/XF Pro
XFe Extracellular Flux Analyzer User Guide (Version 2.6)
XFe and XF Pro Kits – User Guides
Agilent Seahorse XF Real-Time ATP Rate Assay Kit
Agilent Seahorse XF T Cell Metabolic Profiling Kit
Seahorse XF Substrate Oxidation Stress Test Kits User Guide
Agilent Seahorse XF Palmitate-BSA FAO Substrate Quickstart Guide
Agilent Seahorse XF Cell Mito Stress Test Kit
Agilent Seahorse XF Glycolytic Rate Assay Kit
Agilent Seahorse XF Mito Fuel Flex Test Kit
Agilent Seahorse XF Palmitate-BSA FAO Substrate Guide
Agilent Seahorse XF Plasma Membrane Permeabilizer (PMP) Guide
Agilent Seahorse XF Glycolysis Stress Test Kit
XF Pro Specific Kits – User Guides (Only for use with XF Pro)
Agilent Seahorse XF Mito Tox Assay Kit User Guide
Site Preparation Checklists
XFe96 Site Preparation Checklist
XFe24 Site Preparation Checklist
XF Pro Site Preparation Checklist
Normalization – User Guides
Seahorse XF Imaging and Normalization System XFe24 Hoechst33342 Staining Optimization Guidelines
Hydration – User Guides
Loading Injection Ports – User Guides
Loading the Agilent Seahorse XFe96/XF Pro Sensor Cartridge Injection Ports
Loading the Agilent Seahorse XFe24 Sensor Cartridge Injection Ports
Wave Software
Agilent Seahorse XFe96 and XFe24 Analyzers (For use with Wave Desktop 2.4) User Guide
Wave 2.6 User Guide For use with Wave Desktop software and Agilent Seahorse XFe Analyzers
Running Wave Desktop 2.6 on a Mac
Download Hypoxia License Installer
Wave Pro Software
Agilent Seahorse Wave Pro & XF Pro Controller For use with Agilent Seahorse XF Pro Analyzers
Agilent Seahorse XF Pro Controller ReadMe Installation Guide
Agilent Seahorse XF Pro Controller Network Setup Guideline
The Data Quality Analysis View in Agilent Seahorse Wave Pro and Agilent Seahorse Analytics software
Automation Design Document for the Agilent Seahorse XF Pro Analyzer
Articles and Whitepapers:
Agilent Seahorse Analytics Security Version 2.0.1
Improving Quantification of Cellular Glycolytic Rate Using Agilent Seahorse XF Technology
Quantifying Cellular ATP Production Rate Using Agilent Seahorse XF Technology
Technical Overviews
3T3-L1 Pre-Adipocyte Differentiation
Automation Design Document for the Agilent Seahorse XF Pro Analyzer
Conducting an XFe Assay in an Hypoxia Chamber at ≥3% O² *
Evaluation of Edge Effect & Evaporation from Agilent Seahorse XF Pro M Culture Plates
Explore Metabolism Where You Couldn’t Before with Seahorse XF HS Mini Analyzer
Hoechst Staining Optimization Technical Guidelines - XFe24 Analyzer
Hoechst Staining Optimization Technical Guidelines - XFe96 Analyzer
Normalization Technical Guidelines
Islet Assay Using the Agilent Seahorse XF24 Islet Capture Microplate
Methods and strategies for normalizing XF metabolic data to cellular parameters
Modifying Assay Guides for use with Agilent Seahorse XFe24 and XF24 Analyzers
Screening for Mitochondrial Toxicity - A Protocol for the isolation of mitochondria and measurement
Using PMP to Measure Mitochondrial ETC Complex Activity in Limited Biomaterials
Using PMP to Measure Mitochondrial ETC Complex Activity in Limited Biomaterials
Using the XF Plasma Membrane Permeabilizer (PMP) to Measure Substrate Oxidation
Validation of a Six-hour Seahorse XF assay on the Agilent Seahorse XF Pro analyzer
Posters
Changes In Metabolic Phenotype And Cellular ATP Production During CD4+ T Cell Activation
Characterization of fuel dependencies in multidrug resistant breast cancer cells
A Novel Solution to Better Understand the Link Between T Cell Metabolism and Function
Quality Assessment of Metabolic Fitness during T Cell and NK Cell Expansion
Determining Mechanisms of Mitochondrial Toxicity using Agilent Seahorse XF Technology
Determining the Metabolic Consequences of Pharmacological Modulation of T Cell Activation
Metabolic discovery platform:“Seahorse-Bravo”
Application Notes
Application Notes (overall link)
A Customized XF Workflow for Detection and Characterization of Mitochondrial Toxicity
Assessing functional metabolism using cells sourced from blood
Assessing mitochondrial dysfunction in primary cardiomyocytes
Assessing NK Cell Bioenergetic Metabolism Using Extracellular Flux Analysis
Assessing T Cell Bioenergetic Poise and Spare Respiratory Capacity Using Extracellular Flux Analysis
Assessing the impact of autophagy on cellular metabolism
Assessing therapies for traumatic brain or spinal cord injuries
Bioenergetic analysis of primary neuronal deficiencies
Bioenergetic analysis of suspension cells: hematopoietic stem cells and lymphocytes
Cell-Based Screening for Drug‑Induced Mitochondrial Toxicity
Defining metabolic phenotypes in cancer cells
Determining Metabolic Switch in Induced Pluripotent Stem Cells (iPSCs)
Direct Measurements of Cellular Metabolism for Identification of Mitochondrial Drug Targets
Evaluating changes in cell metabolism in neuroimmune and neuropsychiatric disorders
Functional validation of Genomic and Proteomic data
Identifying Metabolic Switches Using an Agilent Seahorse XFe Analyzer in Hypoxia
Measurement of Mitochondrial Toxicity Using Primary Hepatocytes
Measuring bioenergetic profiles of human adipocytes
Measuring functional metabolism of brite adipocytes
Measuring glycolysis and oxidative metabolism in cancer cells
Measuring glycolytic function in cells
Measure Mitochondrial Respiration in Skeletal Muscle Fibers with a Seahorse Analyzer
Measuring the metabolic switch in cancer cells
Metabolic Pathway Determination Using the Seahorse XF24 Analyzer
Normalization of Agilent Seahorse XF Data by In-situ Cell Counting Using a BioTek Cytation 5
Quantitative Analysis of Neutrophil Activation Using Agilent Seahorse XF Technology
Rapid Bioenergetic Functional Screening of Anticancer Drug Candidates
Real Time Discrimination of Inflammatory Macrophage Activation Using Agilent Seahorse XF Technology
XF Data Normalization by the Agilent Seahorse XF Imaging and Normalization System
Quick Reference Guides
Quick Reference Guide: Calculating Proton Efflux Rate (PER) Data
Agilent Seahorse XF Buffer Factor Protocol Quick Reference Guide
Informative and Instructional Videos
Cell Analysis Video Library (Overall)
Analyze Agilent Seahorse XF Real-Time ATP rate assay data using Agilent Seahorse Analytics
How to accelerate your analysis workflow with Agilent Seahorse Analytics
How to transform data into insight with Agilent Seahorse Analytics
How to keep your data organized and collaborate more efficiently with Agilent Seahorse Analytics
Seahorse XF Mito Tox Assay with Seahorse Analytics
Analyze Agilent Seahorse XF Substrate Oxidation Stress Test data using Agilent Seahorse Analytics
Bravo Automation-XF Assay Demonstration
Bravo Automation-XF Assay Streamline
Cell Analysis Normalization System Overview
How to analyze XF neutrophil activation result data
How to accelerate your analysis workflow with Agilent Seahorse Analytics
How to keep your data organized and collaborate more efficiently with Agilent Seahorse Analytics
How to transform data into insight with Agilent Seahorse Analytics
Measuring Cell Metabolism in Transient Microchamber
Seahorse XF Mito Tox Assay with Seahorse Analytics
Seahorse Analyzer Multi Port Drug Injection
Seeding Cell Culture Microplates
Webinars
Webinars (overall link)
Multiplexed Screening of Drug-Induced Mitochondrial Dysfunction and Cytotoxicity
Report Generators – Single and Multi-file (if available)
Seahorse XF Cell Mito Stress Test Report Generators
Seahorse XF Glycolytic Rate Assay Report Generators
Seahorse XF Mito Fuel Flex Test Report Generator
Seahorse XF Real-Time ATP Rate Assay Report Generators
Soluble Sensors
Overall:
MitoXpress Xtra Oxygen Consumption Assay Plate Reader compatibility chart
Ph-Xtra Glycolysis Assay Plate Reader compatibility chart
MitoXpress Intra Plate Reader Compatibility Guide
Instrument Setup Guides – MitoXpress Xtra Oxygen Consumption Assay Setup Guides
MitoXpress Xtra Oxygen Consumption Assay Instrument setup guide for Perkin Elmer plate readers
MitoXpress Xtra Oxygen Consumption Assay Instrument setup guide for Tecan plate readers
MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for BMG Plate Readers
MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for Molecular Devices Plate Readers
MitoXpress Xtra Oxygen Consumption Assay Instrument Set Up Guide for BioTek Plate Readers
Instrument Setup Guides – pH-Xtra Glycolysis Assay Setup Guides
pH-Xtra Glycolysis Assay Instrument setup guide for Perkin Elmer plate readers
pH-Xtra Glycolysis Assay Instrument setup guide for Tecan plate readers
pH-Xtra Glycolysis Assay Instrument Set Up Guide for BioTek Plate Readers
pH-Xtra Glycolysis Assay Instrument Set Up Guide for Molecular Devices Plate Readers
pH-Xtra Glycolysis Assay Instrument Set Up Guide for BMG Plate Readers
User Manuals
Agilent pH-Xtra Glycolysis Assay
Agilent MitoXpress Xtra Oxygen Consumption Assay (HS Method)
Agilent MitoXpress Intra Intracellular Oxygen Assay
Application Notes
Assessing the Impact of Drug Treatment on Cardiomyocyte Function
Metabolic Profiling of Cells in 3D Cultures using MitoXpress Xtra and pH-Xtra Assays
Screening for Drug-Induced Mitochondrial Dysfunction Using MitoXpress Xtra on Isolated Mitochondria
Software
Agilent MitoXpress & pH-Xtra Data Visualization Tool User Manual
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