Courtney Watts
kristen.sensabaughWhat calculations are performed on the data in this visualization tool? Parameter equations are described in Table 1 on page 8 of the user guide, in the “Calculations Performed” on page 8 section.
How do I remove outlier wells in the Data Visualization Tool? See the “Exclude Outliers/Groups from Analysis” on page 42 for more information.
Can I use any file format (.xml/ csv/ .txt) exported from the plate reader for importing into this Tool? No, only the file formats described in the “Export Data from Plate Reader Software” on page 17 section are compatible with this Data Visualization Tool. If you are having difficulties importing the recommended file format for import, please contact Agilent Cell Analysis Technical Support.
There is a large drop in fluorescence signal in the beginning of my results what do I do? This decrease in signal is due to temperature equilibration of the plate, and data illustrating this effect should not be used for slope analysis purposes. We recommend moving the time selection to calculate slope after temperature equilibration effect has plateaus. Please see the user guide for more information on plate Temperature equilibration, and steps to reduce it.
How do I choose the linear portion of the signal curves? Use the time selection values to select for the portion of the signal curves you wish to be included in the analysis. Once chosen, review the R2 values recorded in the Summary Slope table to give you an indication of the linearity of the Slope calculation. Based on these values, the time selection can be adjusted to further improve the R2 value for a given data set as required.
More FAQs: Cell Analysis FAQs and Troubleshooting
Keywords: DVT, Data Visualization Tool, MitoXPress Xtra, pH-Xtra, Parameter Equations, Signal Curves, Outlier, Soluble Sensors, FAQ