Agilent Community
Agilent Community
  • User
  • Site
  • Search the Community
  • User
Cell Analysis
  • Technical Areas
Cell Analysis
Wiki Agilent MitoXpress Xtra and pH-Xtra Assay Data Visualization Tool User Guide FAQs
  • Announcements
  • Forum
  • Files
  • Wiki
  • More
  • Cancel
  • Collection of Cell Analysis Resources
  • Cell Analysis FAQs and Troubleshooting
  • Agilent Cell Analysis Video Library
  • Cell Analysis
  • Agilent MitoXpress Intra Intracellular Oxygen Assay FAQs
  • Agilent MitoXpress Xtra and pH-Xtra Assay Data Visualization Tool User Guide FAQs
  • Agilent MitoXpress Xtra Oxygen Consumption Assay (HS Method) FAQs
  • Agilent pH-Xtra Glycolysis Assay FAQs
  • Agilent Seahorse Calibration Error Troubleshooting Tips
  • Agilent Seahorse Error Message: Door Not Closing Troubleshooting Tips
  • Agilent Seahorse Error on Wave desktop: “Instrument not installed”: Troubleshooting Tips
  • Agilent Seahorse Error Unloading Cartridge Troubleshooting Tips
  • Agilent Seahorse File Save Error: “File cannot be saved”: Troubleshooting Tips
  • Agilent Seahorse Group Name(s) from Wave Don’t Match Excel: Troubleshooting Tips
  • Agilent Seahorse Licensing Issue with Software: Troubleshooting Tips
  • Agilent Seahorse Mito Fuel Flex Report Generator FAQs
  • Agilent Seahorse Mito Stress Test Single-File and Mulit-File XF Report Generator FAQs
  • Agilent Seahorse Motor error/plate tray immovable: Troubleshooting Tips
  • Agilent Seahorse Plate error: Plate Not Compatible with Assay: Troubleshooting Tips
  • Agilent Seahorse Plate error: “This cell plate is not assigned to this sensor cartridge.  Please replace the cell plate and try again”: Troubleshooting Tips
  • Agilent Seahorse Prism Error when opening .Pzfx file exported from Wave Software: Troubleshooting Tips
  • Agilent Seahorse Wave Crashes When “Design” Clicked: Troubleshooting Tips
  • Agilent Seahorse Wave Error exporting to GraphPad Prism: Troubleshooting Tips
  • Agilent Seahorse Wave File Save Error: “We cannot run this experiment.  Some groups have the same name”: Troubleshooting Tips
  • Agilent Seahorse Wave Hypoxia Data Does Not Recalculate after Excluding and then Reincluding the Sulfite Group: Troubleshooting Tips
  • Agilent Seahorse Wave Software Crash or Freeze: Troubleshooting Tips
  • Agilent Seahorse XF Cell Energy Phenotype Test Kit FAQs
  • Agilent Seahorse XF Cell Energy Phenotype Test Report Generator FAQs
  • Agilent Seahorse XF Glycolytic Rate Assay Kit FAQs
  • Agilent Seahorse XF Glycolytic Rate Assay Single-File and Multi-File Report Generator FAQs
  • Agilent Seahorse XF Imaging and Normalization FAQs
  • Agilent Seahorse XF Mito Fuel Flex Test Kit FAQs
  • Agilent Seahorse XF Real-Time ATP Rate Assay Kit FAQs
  • Agilent Seahorse XF Real-Time ATP Rate Assay Report Generator FAQs
  • Agilent Seahorse XFp: Cartridge failed to eject after assay: Troubleshooting Tips
  • Agilent Seahorse XFp: Unable to set up wireless connection with adapter: Troubleshooting Tips
  • Agilent Seahorse: Unable to open Wave Software/data files, Unable to save data files/ Unable to view all assay templates: Troubleshooting Tips
  • Seahorse Barcode-Related Error: Troubleshooting Tips
Still Need Help?

Post your question in our User Forum or Contact Support.

Agilent MitoXpress Xtra and pH-Xtra Assay Data Visualization Tool User Guide FAQs

Created by Courtney Watts Courtney Watts over 2 years ago | Last modified by kristen.sensabaugh kristen.sensabaugh over 1 year ago

What calculations are performed on the data in this visualization tool? Parameter equations are described in Table 1 on page 8 of the user guide, in the “Calculations Performed” on page 8 section.

 

How do I remove outlier wells in the Data Visualization Tool? See the “Exclude Outliers/Groups from Analysis” on page 42 for more information.

 

Can I use any file format (.xml/ csv/ .txt) exported from the plate reader for importing into this Tool? No, only the file formats described in the “Export Data from Plate Reader Software” on page 17 section are compatible with this Data Visualization Tool. If you are having difficulties importing the recommended file format for import, please contact Agilent Cell Analysis Technical Support.

 

There is a large drop in fluorescence signal in the beginning of my results what do I do? This decrease in signal is due to temperature equilibration of the plate, and data illustrating this effect should not be used for slope analysis purposes. We recommend moving the time selection to calculate slope after temperature equilibration effect has plateaus. Please see the user guide for more information on plate Temperature equilibration, and steps to reduce it.

 

How do I choose the linear portion of the signal curves? Use the time selection values to select for the portion of the signal curves you wish to be included in the analysis. Once chosen, review the R2 values recorded in the Summary Slope table to give you an indication of the linearity of the Slope calculation. Based on these values, the time selection can be adjusted to further improve the R2 value for a given data set as required.

 

More FAQs: Cell Analysis FAQs and Troubleshooting

 

Keywords: DVT, Data Visualization Tool, MitoXPress Xtra, pH-Xtra, Parameter Equations, Signal Curves, Outlier, Soluble Sensors, FAQ

  • signal curves
  • data visualization tool
  • outlier
  • Soluble Sensors
  • mitoxpress xtra
  • parameter equations
  • dvt
  • ph-xtra
  • faq
  • Share
  • History
  • More
  • Cancel
Anonymous
Related

Agilent Community Feedback

Agilent Community Feedback

×
We are glad this was helpful! We are sorry this was not helpful. If you still need assistance please create a community post or contact support. To help us improve, please provide any additional feedback. For full details of how we will treat your information, please view our privacy policy.
Submit Cancel
Submit Cancel
Recommended
Privacy Statement
Terms of Use
Contact Us
Site Help