Agilent Community
Agilent Community
  • User
  • Site
  • Search the Community
  • User
Cell Analysis
  • Technical Areas
Cell Analysis
Wiki Agilent pH-Xtra Glycolysis Assay FAQs
  • Announcements
  • Forum
  • Files
  • Wiki
  • More
  • Cancel
  • Collection of Cell Analysis Resources
  • Cell Analysis FAQs and Troubleshooting
  • Agilent Cell Analysis Video Library
  • Cell Analysis
  • Agilent MitoXpress Intra Intracellular Oxygen Assay FAQs
  • Agilent MitoXpress Xtra and pH-Xtra Assay Data Visualization Tool User Guide FAQs
  • Agilent MitoXpress Xtra Oxygen Consumption Assay (HS Method) FAQs
  • Agilent pH-Xtra Glycolysis Assay FAQs
  • Agilent Seahorse Calibration Error Troubleshooting Tips
  • Agilent Seahorse Error Message: Door Not Closing Troubleshooting Tips
  • Agilent Seahorse Error on Wave desktop: “Instrument not installed”: Troubleshooting Tips
  • Agilent Seahorse Error Unloading Cartridge Troubleshooting Tips
  • Agilent Seahorse File Save Error: “File cannot be saved”: Troubleshooting Tips
  • Agilent Seahorse Group Name(s) from Wave Don’t Match Excel: Troubleshooting Tips
  • Agilent Seahorse Licensing Issue with Software: Troubleshooting Tips
  • Agilent Seahorse Mito Fuel Flex Report Generator FAQs
  • Agilent Seahorse Mito Stress Test Single-File and Mulit-File XF Report Generator FAQs
  • Agilent Seahorse Motor error/plate tray immovable: Troubleshooting Tips
  • Agilent Seahorse Plate error: Plate Not Compatible with Assay: Troubleshooting Tips
  • Agilent Seahorse Plate error: “This cell plate is not assigned to this sensor cartridge.  Please replace the cell plate and try again”: Troubleshooting Tips
  • Agilent Seahorse Prism Error when opening .Pzfx file exported from Wave Software: Troubleshooting Tips
  • Agilent Seahorse Wave Crashes When “Design” Clicked: Troubleshooting Tips
  • Agilent Seahorse Wave Error exporting to GraphPad Prism: Troubleshooting Tips
  • Agilent Seahorse Wave File Save Error: “We cannot run this experiment.  Some groups have the same name”: Troubleshooting Tips
  • Agilent Seahorse Wave Hypoxia Data Does Not Recalculate after Excluding and then Reincluding the Sulfite Group: Troubleshooting Tips
  • Agilent Seahorse Wave Software Crash or Freeze: Troubleshooting Tips
  • Agilent Seahorse XF Cell Energy Phenotype Test Kit FAQs
  • Agilent Seahorse XF Cell Energy Phenotype Test Report Generator FAQs
  • Agilent Seahorse XF Glycolytic Rate Assay Kit FAQs
  • Agilent Seahorse XF Glycolytic Rate Assay Single-File and Multi-File Report Generator FAQs
  • Agilent Seahorse XF Imaging and Normalization FAQs
  • Agilent Seahorse XF Mito Fuel Flex Test Kit FAQs
  • Agilent Seahorse XF Real-Time ATP Rate Assay Kit FAQs
  • Agilent Seahorse XF Real-Time ATP Rate Assay Report Generator FAQs
  • Agilent Seahorse XFp: Cartridge failed to eject after assay: Troubleshooting Tips
  • Agilent Seahorse XFp: Unable to set up wireless connection with adapter: Troubleshooting Tips
  • Agilent Seahorse: Unable to open Wave Software/data files, Unable to save data files/ Unable to view all assay templates: Troubleshooting Tips
  • Seahorse Barcode-Related Error: Troubleshooting Tips
Still Need Help?

Post your question in our User Forum or Contact Support.

Agilent pH-Xtra Glycolysis Assay FAQs

Created by Courtney Watts Courtney Watts over 2 years ago | Last modified by kristen.sensabaugh kristen.sensabaugh over 1 year ago

What do I do if I cannot detect any signal in wells containing cells and pH-Xtra (or I can detect a signal but the slope (rate) appears very low)?

Check correct Instrument Settings (“Appendix A - Instrument Settings” on page 29)- Perform Signal Optimization - Include Signal Controls as described previously - Increase cell density. Check pH of prewarmed Respiration Buffer and correct as necessary, as pH can drift over time. If tested and not resolved, contact www.agilent.com.

 

What do I do if I can detect a signal in wells containing cells and pH-Xtra, but the slope (rate) falls initially or is variable from well to well?

Check cell seeding and pipetting consistency; increase cell density; ensure plate, instrument, and all culture media and stock solutions are prewarmed at 37 °C before use; reduce plate preparation times; reduce assay volume to 60 μL.

 

More FAQs: Cell Analysis FAQs and Troubleshooting

 

Keywords: pH-Xtra, pH Xtra Glycolysis Assay, Signal, Slope, Signal Optimization, Soluble Sensors, FAQ

  • ph-xtra glycolysis assay
  • Soluble Sensors
  • signal optimization
  • slope
  • signal
  • ph-xtra
  • faq
  • Share
  • History
  • More
  • Cancel
Anonymous
Related

Agilent Community Feedback

Agilent Community Feedback

×
We are glad this was helpful! We are sorry this was not helpful. If you still need assistance please create a community post or contact support. To help us improve, please provide any additional feedback. For full details of how we will treat your information, please view our privacy policy.
Submit Cancel
Submit Cancel
Recommended
Privacy Statement
Terms of Use
Contact Us
Site Help