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All recent questions and discussions
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Not Answered
What scale factor value I must use to normalize data using protein concentration?
0
70
views
2
replies
Latest
3 days ago
by
ValJH
Not Answered
DMEM, low glucose, pyruvate, no glutamine, no phenol red
0
70
views
1
reply
Latest
5 days ago
by
Courtney Watts
Not Answered
How to measure Mitochondrial acidification from Glycolytic Rate Assay?
0
80
views
1
reply
Latest
19 days ago
by
Courtney Watts
Not Answered
Oligomycin is not working
0
240
views
4
replies
Latest
1 month ago
by
Chanyoung
Not Answered
C2C12 Cell detachment from 96 well plate
0
275
views
5
replies
Latest
1 month ago
by
Courtney Watts
Answered
Barcode reader offline for Seahorse XFe96
0
191
views
1
reply
Latest
1 month ago
by
Courtney Watts
Answered
Low BASAL OCR
+1
486
views
1
reply
Latest
2 months ago
by
Courtney Watts
Answered
non-mitochondrial consumption value for Ctrl vehicle is 6.20 to -23.6
+1
944
views
3
replies
Latest
2 months ago
by
Courtney Watts
Answered
Oligo/FCCP/Rot/AA drug recommended conc for human melanoma cell lines (A375 and SKMEL5)
+1
781
views
2
replies
Latest
2 months ago
by
Courtney Watts
Answered
Inter-Plate Variation
+1
532
views
3
replies
Latest
2 months ago
by
Courtney Watts
Answered
No response to oligomycin during glycolysis stress test with intestinal organoids
+1
477
views
3
replies
Latest
2 months ago
by
Courtney Watts
Answered
Agilent Seahorse XF RPMI Medium
+1
468
views
5
replies
Latest
2 months ago
by
Courtney Watts
Answered
Low OCR values for ATP rate assay kit on IPEC-J2 cells
+1
1077
views
2
replies
Latest
2 months ago
by
Courtney Watts
Answered
Is it acceptable to substitute EDTA for EGTA when preparing the Mitochondrial Assay Solution (MAS, 1X)?
+1
304
views
1
reply
Latest
2 months ago
by
Courtney Watts
Answered
Seahorse on isolated mitochondria
+1
1286
views
2
replies
Latest
2 months ago
by
Gaurav Verma
Answered
What solvent for respiration reagent on isolated mitochondria assays?
+1
193
views
1
reply
Latest
3 months ago
by
Courtney Watts
Answered
Is there have Finished kit for Coupling or Electron Flow measurement in isolated mitochondrial with XFe24 or XFe96?
+1
253
views
3
replies
Latest
3 months ago
by
Courtney Watts
Answered
OD measurement in BioTek Epoch 2 Microplate Spectrophotometer
+1
352
views
1
reply
Latest
3 months ago
by
Courtney Watts
Answered
Extracellular Flux Cartridge
+1
704
views
5
replies
Latest
4 months ago
by
Courtney Watts
Answered
C02 value for ATP calculations
+1
1091
views
8
replies
Latest
4 months ago
by
Courtney Watts
Answered
Drug injection fail
+1
5736
views
10
replies
Latest
5 months ago
by
Courtney Watts
Answered
Non-glycolytic acidification: last measure before glucose, or lowest ECAR after 2-DG?
+2
5124
views
9
replies
Latest
6 months ago
by
Courtney Watts
Answered
Primary human skeletal muscle cells
+1
533
views
1
reply
Latest
7 months ago
by
Courtney Watts
Answered
What combination of substrate I need to use on my isolated mitochondria on seahorse plate to keep them respiring?
+1
291
views
3
replies
Latest
7 months ago
by
Courtney Watts
Answered
Seahorse XF Palmitate Oxidation Stress Test in B16F10 cells
+1
836
views
2
replies
Latest
9 months ago
by
yadachi
Answered
How to know and detect signal not use the RTCA?
+1
807
views
1
reply
Latest
over 1 year ago
by
Courtney Watts
Answered
Barcode errors - help?
+1
1258
views
1
reply
Latest
over 1 year ago
by
Courtney Watts
Answered
Seahorse plate orientation and data analysis
+1
1318
views
2
replies
Latest
over 1 year ago
by
TEve
Answered
Mito Fuel Flex Test
+1
986
views
1
reply
Latest
over 1 year ago
by
Courtney Watts
Answered
Cell metrics export
+1
2218
views
2
replies
Latest
over 1 year ago
by
WJCannan
Answered
How to export Bam files from Agilent Surecall software
+1
2887
views
2
replies
Latest
over 1 year ago
by
Tom1884it
Answered
plate coating
+1
2724
views
3
replies
Latest
over 1 year ago
by
Courtney Watts
Answered
Glycolytic rate assay with PBMCs
+1
1744
views
1
reply
Latest
over 1 year ago
by
Courtney Watts
Answered
Different kits at the same time
+1
2039
views
1
reply
Latest
over 1 year ago
by
Courtney Watts
Answered
getting most of ECAR data from a mito-stress test
+1
5659
views
1
reply
Latest
over 2 years ago
by
Courtney Watts
Answered
Seahorse Xfe96 mito stress maximum OCR
+1
3406
views
3
replies
Latest
over 2 years ago
by
Courtney Watts
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