Is it acceptable to substitute EDTA for EGTA when preparing the Mitochondrial Assay Solution (MAS, 1X)？

Question

Hi Courtney, 
 Thank you for your response. I have a question about the Mitochondrial Assay Solution (MAS, 1X). When conducting Seahorse experiments with isolated mitochondria, is it acceptable to substitute EDTA for EGTA when preparing the Mitochondrial Assay Solution (MAS, 1X)？ 
 I eagerly await your response. 
 
 Best Regards, 
 LD

Courtney Watts · Accepted Answer

Hi LD, 
 We do not recommending substituting as EGTA has higher affinity for chelating calcium ions and lower affinity for chelating Mg2+ ions compared to EDTA. 
 
 Thank you and please let me know if you have any other questions! 
 Courtney

Is it acceptable to substitute EDTA for EGTA when preparing the Mitochondrial Assay Solution (MAS, 1X)？

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