Drug injection fail

Hi,

This is Xiaofei from Seahorse Tech Support. Could you send me the data files or the pictures to show the drug didn't get injected (if you have) to this email address: cellanalysis.support@agilent.com

Thanks,

Xiaofei Chen, PhD

Technical Support Scientist --- Cell Analysis division

www.Agilent.com

Email: cellanalysis.support@agilent.com

Phone: 800-227-9770--option 3--option 8

I just wanted to follow-up to see if this issue is resolved. Was your instrument repaired, or was there a technical issue with the loading process? If your problem was resolved, please come back to the post and click Verify Answer on the best response so it will make the solution more visible. If you still need help, just let us know and we would be happy to continue working with you.

This happened to me today. I only used 20 wells out of 96 wells plate. I don't know whether that cause the problem.

I have the same problem on the XF HS mini analyser, some drugs are not injected, sometimes it is the A or the B port depending of the run.

Hi Ruohan and Lbmp,

Can you tell me if, when loading the ports, all ports of the series being used were loaded? For example, if using Port A, were all ports (including background and unseeded wells) filled with liquid? Not doing this can result in issues with injections. 

Thank you,

Courtney

Yes all the ports were loaded

Hi,

The tech told me to fill all the wells. I loaded all ports (A,B, and C) in all the wells of the plate, and that solved the problem. 

This means we can't use the partial plate.

Sincerely

Ruohan 

Hi Ruohan,

Thank you for your reply. Correct, if you are using ports A, B, and C, you will want to load all of those ports with either reagent or assay media (for the wells that do not have cells). If this is not done, the instrument cannot build up pressure and inject the reagents properly.  I am sorry about the inconvenience this causes!

Thank you,

Courtney 

Hi Lbmp,

If you could send your data to cellanalysis.support@agilent.com, we can take a look at it and let you know what might be going on!

Thank you,

Courtney

For anyone interested in taking a look at our port loading guides/protocols, please see below:

Loading the Agilent Seahorse XFe96 Sensor Cartridge Injection Ports

Loading the Agilent Seahorse XFe 24/XF24 Sensor Cartridge Injection Ports

Loading the Agilent Seahorse XFp Sensor Cartridge Injection Ports