Oligomycin is not working

Hello, I am trying to measure OCR with FHs74 Int cell line (ATCC) and optimizing the concentrations of Oligomycin, FCCP, and Rotenone/antimycin A.

I confirmed that FCCP works at 1 μM and 5 μM, and Rotenone/antimycin A works at 0.5 μM.

However, Oligomycin does not work at 1.5, 2, 2.5, or 3 μM (the OCR graph line does not decrease after Oligomycin injection).

I don't know how to solve this issue, so I would like to get some advice.

Note: This is an untreated, normal state of FHs74 Int cell line.

I look forward to hearing from you.

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