• State Verified Answer
  • +1 person also asked this people also asked this
  • Replies 1 reply
  • Subscribers 9 subscribers
  • Views 638 views
  • Users 0 members are here
Options
Related

How to measure Mitochondrial acidification from Glycolytic Rate Assay?

CMs

Hi,

I recently performed the Glycolytic Rate Assay and normalized the data using cell counts. I organized the data into two sets: before and after ROT/AA, and post 2-DG.

  1. How do I calculate the mitochondrial acidification rate from this data?
  2. I noticed that I only obtained ECAR values for my conditions, and for some of my conditions, I received PER values, but for others, the values were zero. Could you help me understand why this might be happening?

Thank you.

  • +1

    Hi there,

    Thank you for contacting Cell Analysis Technical Support. Regarding calculations, please see the below chart for the GRA Assay.  Additionally, here is a link to the GRA White Paper.

    For your second question, I would have to take a look at your data. If you could go ahead and send your Wave results file to cellanalysis.support@agilent.com, I would be happy to do this.

    Thank you,

    Courtney

    Courtney Nadeau Watts

    Technical Support Scientist/Remote Engineer

    Cell Analysis Products

Was this helpful?
Privacy Statement
Terms of Use
Contact Us
Site Help