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Seahorse on isolated mitochondria

Gaurav Verma

I am isolating mitochondria from mouse brain and want to run seahorse onto it, could you recommend the best possible protocol for the isolated mitochondria on seahorse XFe96 analyzer?

Looking forward,

Best regards.

Gaurav

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    Dear LD,
    If your experiment requires precise control over calcium levels, EGTA should be preferred due to its higher selectivity for calcium ions. However, if calcium levels are not critical or can be adjusted accordingly, EDTA may be a suitable alternative.

    I am regularly doing seahorse on isolated mitochondria from mice brain and Agilent protocol gave me fabulous results. I used EGTA in my 1X MAS buffer.

    Of note, I am just sharing my experiences, Courtney maybe able to give more insights.

    Cheers,
    Gaurav
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    Dear LD,
    If your experiment requires precise control over calcium levels, EGTA should be preferred due to its higher selectivity for calcium ions. However, if calcium levels are not critical or can be adjusted accordingly, EDTA may be a suitable alternative.

    I am regularly doing seahorse on isolated mitochondria from mice brain and Agilent protocol gave me fabulous results. I used EGTA in my 1X MAS buffer.

    Of note, I am just sharing my experiences, Courtney maybe able to give more insights.

    Cheers,
    Gaurav
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