I work in a small, environmental lab. We test aluminum levels in serum using ICP-MS. We can run batches of 250 samples at a time. We dilute 500 microliters of serum to 5 ml using 0.2% HNO3. We calibrate using 1, 5, 10, and 20 ppb Aluminum std. At the…
I looked at your plot again. That little bounce is barely 0.01pA -- so very tiny! This is down in the lowest possible signals that can be measured with an FID and honestly should not matter as your smallest peaks of interest should be at least 50x to…
Hello Agilent community,
I have recently been 'Recovery Test in GMP' of Openlab 3.4 (Build 3.4.0.242) in a standalone environment using the Windows Hyper-V feature.
During the testing, I found the following two issues
First, a warning message…
We run a lot of our GCs with Polyarcs (methanizers) and FID responses can be theoretically calculated. While upgrading the software and importing chemstation methods, the calibration table did not get imported. If I could manually edit the calibration…
Hello,
For awhile now, I've been having issues with my resolution axis "passing" for masses 25 and 26 in no gas mode. I have done many cleanings and new tuning solutions. Can anyone explain what is causing this continual failing and what I could do…
After a failed methamphetamine quantitation, it was noticed that the methamphetamine peak shape on our GC/FID instruments has been consistently poor or deteriorating through out a run. This has been happening on both of our GC/FID instruments (all 3 columns…
It looks like theres a problem with the back inlet/back autosampler - the gc does not recognize it anymore - but it seems to be defined within the method, meaning it has a inlet temperature ect.
Did you change something regarding the back inlet? or…
We have OpenLab 2.1 (with content manger) running our GC and LC systems. We have successfully been running 2 x GC and 6 x LC systems however this morning on two of the systems we can see the following error:
Acquisition
Failed to connect to the…
Hello, Just to add on to dnprulz 's reply, the expression that he provided will work in OpenLab Custom Calculator and should perform what you are looking to do. However, if you want the pass/fail logic to be set on the report template then you will want…
This Information Applies To: Fragment Analyzer, Femto Pulse, ZAG, and their respective controller software
Issue:
If there is dried gel in the reservoir vent valve of your Agilent Fragment Analyzer, Femto Pulse, or ZAG, cleaning is required. A clogged...
This Information Applies To: 5200, 5300, 5400 Fragment Analyzer, Femto Pulse, Oligo Pro II, and ZAG
Issue:
Adding mineral oil to Agilent parallel capillary electrophoresis DNA marker plates and samples plates prevents evaporation and enables re...
This Information Applies To: 5200/5300/5400 Fragment Analyzer, Femto Pulse, Oligo Pro II, and ZAG
Issue:
Only plasticware from the approved consumables list should be used in the Agilent Parallel Capillary Electrophoresis systems. The use of plasticware...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Question:
Can more than one Agilent 2100 Bioanalyzer instrument be connected to one PC?
Steps to follow:
It is possible to connect a maximum of two Bioanalyzers to one laptop...
This Information Applies To: 2100 Bioanalyzer, Bioanalyzer Chip Priming Station: part number 5065–4401
Maintenance procedures and how frequently they are required :
Procedure
Time interval
Or if this issue occurs
Where to find a detailed...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Issue:
Running the hardware diagnostics on the Agilent 2100 Bioanalyzer system can help to investigate whether the instrument performs as expected.
Steps to follow:
Required...
This Information Applies To: 2100 Bioanalyzer, RNA 6000 Nano kit, RNA 6000 Pico kit, Small RNA kit
Issue:
Is it possible to size RNA on the Agilent 2100 Bioanalyzer system?
Resolution:
Agilent offers total and small RNA assays on the Bioanalyzer...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue:
The Agilent 2100 Bioanalyzer system does not detect a prepared microfluidic chip after it is inserted into the instrument. The interface shows the instrument...
This Information Applies To: 2100 Bioanalyzer, Bioanalyzer chips
Issue:
Can 2100 Bioanalyzer assay chips and reagents be interchanged?
Resolution:
Some assay chips can be used with multiple assays, however some cannot because the chip layout...
This Information Applies To: Agilent MassHunter GC/MS Acquisition software
Issue
The MassHunter software performs several checks during the startup sequence. If it detects a problem, an alert message box will be displayed and the message bar on...