• Created : 
  • Modified : 
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Automated Electrophoresis Systems
  • Component :  Bioanalyzer Systems
  • Product Name :  2100 Bioanalyzer Instrument
  • Task :  Maintenance
Related Articles

  • Reporting Signal-to-Noise in OpenLab ChemStation

    This Information Applies To: Agilent OpenLab ChemStation Content The following procedure shows how to check the signal-to-noise ratio in Agilent OpenLab ChemStation and report the signal-to-noise ratio in classic and intelligent reports. Background Agilent OpenLab ChemStation has the following options to calculate the noise for reporting the signal-to-noise ratio; 6 Sigma : Noise is calculated using six times the standard deviation of the linear regression (6 sigma). USP (according...

  • Signal-to-Noise Calculation in OpenLab ChemStation

    This Information Applies To: Agilent OpenLab ChemStation Issue This article describes the fundamentals of noise determination and calculation in Agilent OpenLab ChemStation to report the signal-to-noise ratio. Tips : To report the signal-to-noise ratio in OpenLab CDS ChemStation Edition, see the article Reporting Signal-to-Noise in OpenLab ChemStation . Resolution Noise Determination and Calculation Noise can be determined from the data point values from a selected...

  • Wavy Baseline on the Agilent Bioanalyzer

    This Information Applies To: 2100 Bioanalyzer, 2100 Expert software Issue The Agilent 2100 Bioanalyzer system electropherograms in the Agilent 2100 Expert software have a wavy, uneven baseline ( Figure 1 ). Figure 1. Wavy, uneven baseline on Bioanalyzer electropherograms The error is most often associated with: Problems during chip priming Dirty electrodes Moisture on the electrodes Sample contamination The sample concentration being higher than the assay's range Liquid spillage...

  • Agilent 2100 Bioanalyzer Error: Run Aborted on Port x, Unusual High or Low Voltage

    This Information Applies To: 2100 Bioanalyzer, 2100 Expert software Issue The Agilent 2100 Bioanalyzer system run ends prematurely and the following error appears in the logbook: Run aborted on port x. Instrument error occurred on port x, Unusual high or low voltage or current was detected during the start phase of the on-chip analysis. The marked wells will indicate the wells on the chip that caused the problem. An example of the error is shown in Figure 1 . The error is most often associated...

  • Troubleshooting Late Migration on the Agilent 2100 Bioanalyzer System

    This Information Applies To: 2100 Bioanalyzer system, 2100 Expert software Issue: How to identify and troubleshoot late migration of the ladder, marker, and sample peaks in data generated on the Agilent 2100 Bioanalyzer system. Background The ladder, marker, and sample peaks in the affected run take considerably longer to migrate compared to the peaks in the demo file of the same assay type. Late migration is usually accompanied by errors, similar to the ones shown in Figure 1 . These...

Maintenance of the Agilent 2100 Bioanalyzer System Chip Priming Station

Answer

This Information Applies To: 2100 Bioanalyzer, Bioanalyzer Chip Priming Station: part number 5065–4401

Maintenance procedures and how frequently they are required:

Procedure Time interval Or if this issue occurs

Where to find a detailed description in the Maintenance and Troubleshooting Guide
Replacing the syringe Usually with each new kit, or at least every three months. However, due to the current unavailability of syringes, only if the seal test fails. Priming performance is not affected as long as the seal test passes. Syringe is broken or seal test fails Page 133 (please note previously mentioned changes)
Cleaning or replacing the syringe adapter Every three months Adapter is clogged with dried gel, or damaged Page 136
Replacing the gasket Every three months Gasket is damaged, torn, or contaminated with dried gel Page 137
Checking the chip priming station for proper seal Every four weeks Gasket, syringe adapter, or syringe was replaced Page 139

Steps to follow:

Please find detailed descriptions for every procedure as referenced earlier in this Agilent 2100 Bioanalyzer System Maintenance and Troubleshooting Guide, pages 131-140. 

Please find all the steps in the following video (Figure 1) or in original size on our webpage via this link:

 

Figure 1. Bioanalyzer Chip Priming Station maintenance

 

 Tip 
Learn how to effectively troubleshoot your Bioanalyzer:

For Research Use Only. Not for use in diagnostic procedures.

PR7000-8082

 
Was this helpful?
Privacy Statement
Terms of Use
Contact Us
Site Help