Agilent Community
Agilent Community
  • User
  • Site
  • Search the Community
  • User
  • Home
  • Technical Areas
    Technical Areas
    • AssayMap
    • Atomic Spectroscopy
    • Automated Electrophoresis
    • Cell Analysis
    • Consumables
    • CrossLab Connect
      CrossLab Connect
      • Service Management
      • Smart Alerts
    • Dissolution
    • GC
    • GC/MS
    • LC
    • LC/MS
    • Life Sciences Reagents
    • Molecular Spectroscopy
      Molecular Spectroscopy
      • Molecular Spectroscopy - UV-Vis & UV-Vis-NIR, FTIR, Raman & Fluorescence
      • 8700 LDIR User Group
      • Transmission Raman User Group
    • Real-Time PCR (qPCR)
    • Sample Preparation
    • Software
      Software
      • Chromatography Software
        OpenLab CDS, OpenLab ChemStation, OpenLab EZChrom
      • Data Management Software
        OpenLab ECM, OpenLab ECM XT, OpenLab Server
      • Mass Spectrometry Software
        Data Analysis, Data Acquisition, Productivity App, WalkUp Software
    • Vacuum
  • Applications Areas
    Applications Areas
    • Basic Research
    • Energy and Chemicals
    • Environmental
    • Food and Agriculture
  • Agilent Knowledge Portal
  • Links
    Links
    • Collection of Support Resources
    • Webinar Notifications
    • Contact Support
    • User Contributions
  • Getting Started
    Community Help and Resources
  • Browse
    Browse
    • People
    • Places
    • Your View
    • Your Community Inbox
Automated Electrophoresis Portal
  • Agilent Knowledge Portal
Automated Electrophoresis Portal
Articles Agilent Bioanalyzer Assay Chip Guide
  • Articles
  • Files
  • Forum
  • Announcements
  • More
  • Cancel
  • Agilent Knowledge Portal
  • Automated Electrophoresis Portal Home
  • Automated Electrophoresis Portal Articles
  • Automated Electrophoresis Portal Files
  • More
  • Cancel
  • Created :  10 Nov 2022
  • Modified :  7 Feb 2023
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Automated Electrophoresis Systems
  • Component :  Bioanalyzer Kits and Reagents Bioanalyzer Systems
  • Product Name :  2100 Bioanalyzer Instrument Bioanalyzer DNA Analysis Bioanalyzer High Sensitivity DNA Analysis Bioanalyzer High Sensitivity RNA Analysis Bioanalyzer RNA Analysis Bioanalyzer Small RNA Analysis
  • Task :  Best Practices Operation
Related Articles
  • Finding Declarations of Conformity, Certificates of Conformance and Safety Data Sheets for Agilent Bioanalyzer Reagents

    This Information Applies To: 2100 Bioanalyzer reagents and chips Issue Agilent publishes the declarations of conformity (DOC) for Agilent Bioanalyzer reagents and certificates of conformance (COC) for Bioanalyzer microfluidics chips on Agilent.com . The DOC and COC have been published on Agilent.com for every lot produced since May 2021. To improve the reagents environmental footprint, Agilent removed the hard-copy of the DOC, COC, and the document providing the link to the safety data sheets...
  • Wavy Baseline on the Agilent Bioanalyzer

    This Information Applies To: 2100 Bioanalyzer, 2100 Expert software Issue The Agilent 2100 Bioanalyzer system electropherograms in the Agilent 2100 Expert software have a wavy, uneven baseline ( Figure 1 ). Figure 1. Wavy, uneven baseline on Bioanalyzer electropherograms The error is most often associated with: Problems during chip priming Dirty electrodes Moisture on the electrodes Sample contamination The sample concentration being higher than the assay's range Liquid spillage...
  • Agilent 2100 Bioanalyzer System Chip not Detected

    This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software Issue: The Agilent 2100 Bioanalyzer system does not detect a prepared microfluidic chip after it is inserted into the instrument. The interface shows the instrument icon (or the last chip type used). However, the start run checklist shows that a chip is not detected (see Figure 1 ). Figure 1 . Chip not detected (see red arrow) Not detecting a chip can indicate an issue with the Bioanalyzer-PC connection...
  • Maintenance of the Agilent 2100 Bioanalyzer System Chip Priming Station

    This Information Applies To: 2100 Bioanalyzer, Bioanalyzer Chip Priming Station: part number 5065–4401 Maintenance procedures and how frequently they are required : Procedure Time interval Or if this issue occurs Where to find a detailed description in the Maintenance and Troubleshooting Guide Replacing the syringe Usually with each new kit, or at least every three months. However, due to the current unavailability of syringes, only if the seal test fails. Priming performance...
  • Agilent 2100 Bioanalyzer Error: Run Aborted on Port x, Unusual High or Low Voltage

    This Information Applies To: 2100 Bioanalyzer, 2100 Expert software Issue The Agilent 2100 Bioanalyzer system run ends prematurely and the following error appears in the logbook: Run aborted on port x. Instrument error occurred on port x, Unusual high or low voltage or current was detected during the start phase of the on-chip analysis. The marked wells will indicate the wells on the chip that caused the problem. An example of the error is shown in Figure 1 . The error is most often associated...

Agilent Bioanalyzer Assay Chip Guide

Answer

 

This Information Applies To: 2100 Bioanalyzer, Bioanalyzer chips


Issue:

Can 2100 Bioanalyzer assay chips and reagents be interchanged?


Resolution:

Some assay chips can be used with multiple assays, however some cannot because the chip layout and assay script are different.

 Caution 
Caution: Agilent does not guarantee the proper performance of runs when chips and reagents are interchanged inappropriately. 

Table 1 shows the range of Agilent Bioanalyzer assay chips available.  

DNA
RNA
Protein

Table 1. Bioanalyzer chip guide

Table 2 shows the chips and the corresponding assays available in the 2100 Expert software. 

Chip type Assay
DNA

DNA 1000

DNA 7500

DNA 12000

High Sensitivity DNA

High Sensitivity DNA

RNA Nano

Eukaryotic Total RNA Nano

Prokaryotic Total RNA Nano

Plant RNA Nano 

mRNA Nano

DV200 RNA Nano

RNA Pico

Eukaryotic Total RNA Pico

Prokaryotic Total RNA Pico

Plant RNA Pico

mRNA Pico

DV200 RNA Pico

Small RNA

Small RNA

Protein

Protein 80

Protein 230

High Sensitivity Protein

High Sensitivity Protein 250

Table 2. Bioanalyzer assay chip summary

 Tip 
Learn how to effectively use and troubleshoot your Bioanalyzer System: 2100 Bioanalyzer System Maintenance and Troubleshooting Guide 

 

 

For Research Use Only. Not for use in diagnostic procedures.

PR7000-8190

 
Was this helpful?
  • Yes
  • No
Thank you for your feedback!

Agilent Community Feedback

Agilent Community Feedback

×
We are glad this was helpful! We are sorry this was not helpful. If you still need assistance please create a community post or contact support. To help us improve, please provide any additional feedback. For full details of how we will treat your information, please view our privacy policy.
Submit Cancel
Submit Cancel
Privacy Statement
Terms of Use
Contact Us
Site Help