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Articles How to Use Mineral Oil to Prevent Evaporation on Agilent Parallel Capillary Electrophoresis Systems
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  • Created :  10 Nov 2022
  • Modified :  7 Feb 2023
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Automated Electrophoresis Systems
  • Component :  Femto Pulse Systems Fragment Analyzer Systems Oligo Pro II Systems ZAG DNA Analyzer Systems
  • Product Name :  5200 Fragment Analyzer System 5300 Fragment Analyzer System 5400 Fragment Analyzer System Femto Pulse System Oligo Pro II System ZAG DNA Analyzer system
  • Task :  Best Practices Operation
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How to Use Mineral Oil to Prevent Evaporation on Agilent Parallel Capillary Electrophoresis Systems

Answer

This Information Applies To: 5200, 5300, 5400 Fragment Analyzer, Femto Pulse, Oligo Pro II, and ZAG


Issue:

Adding mineral oil to Agilent parallel capillary electrophoresis DNA marker plates and samples plates prevents evaporation and enables re-use of the plates. 

 Caution 
Caution: Agilent has not validated adding mineral oil to RNA plates. It is used at the customers own risk. 

Steps to follow:

 Tip 
Tip: Agilent recommends using mineral oil part number FS-SMO15 to overlay sample or marker plates when appropriate.
  1. Add one drop of mineral oil into each well of the prepared marker or sample plate directly from the mineral oil drop bottle. One drop is equivalent to a volume of 20 µL. See the Quick Guide supplied with kit you are running for information on how soon the mineral oil covered plate should be used.
Tip

Learn how to effectively operate your Agilent Parallel Capillary Electrophoresis system: 
5200, 5300, and 5400 Fragment Analyzer System Manual
Femto Pulse User Manual
Oligo Pro II User Manual 
ZAG DNA Analyzer System Manual 

For Research Use Only. Not for use in diagnostic procedures.

PR7000-8119

 
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