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Articles Can the Agilent 2100 Bioanalyzer be Used to Determine Size of RNA?
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  • Created :  10 Nov 2022
  • Modified :  7 Feb 2023
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Automated Electrophoresis Systems
  • Component :  Bioanalyzer Kits and Reagents Bioanalyzer Systems
  • Product Name :  2100 Bioanalyzer Instrument Bioanalyzer High Sensitivity RNA Analysis Bioanalyzer RNA Analysis Bioanalyzer Small RNA Analysis
  • Task :  Best Practices Operation
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Can the Agilent 2100 Bioanalyzer be Used to Determine Size of RNA?

Answer

This Information Applies To: 2100 Bioanalyzer, RNA 6000 Nano kit, RNA 6000 Pico kit, Small RNA kit 


Issue: 

Is it possible to size RNA on the Agilent 2100 Bioanalyzer system?


Resolution: 

Agilent offers total and small RNA assays on the Bioanalyzer. 

There are no sizing specifications available for total RNA assays as the assays are not developed or validated for sizing accuracy and precision. However, given that ladder consists of RNA fragments from 200 nt to 6000 nt, it is possible to obtain sizing information in that range. Agilent offers RNA assays that allow characterization of a variety of RNA sample types and estimation of their concentrations. Please refer to our datasheet for the RNA Kits.

Small RNA assay specifications do provide a sizing range. However, like total assay, no sizing accuracy or resolution information is available.

 

 Tip 
Learn how to effectively use: 
Agilent RNA 6000 Nano Kit Guide
Agilent RNA 6000 Pico Kit Guide
 
Agilent Small RNA Kit Guide

For Research Use Only. Not for use in diagnostic procedures

PR7000-8668

Note: Remember to add DE, or RA number and Keywords in the Options tab

 
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