Hello
I need to do an LC/MSMS method for quantification on a compound that doesn't ionize into [M+H]+ (barely observed) but only into [M-H2O+H]+
Is there a problem with doing MS/MS on a [M-H2O+H]+ parent ion ?
(PS: negative ionization is not an…
Hi again Raina,
as I already told you this cannot be done with just one column, especially with the CO2 concentration up to 20% and water included and the necessity to analyze H2. This is an application we normally build an analyzer for. You need at…
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Operating like this puts sub-optimal flow into the GCMS ion source of more than 2 ml/min. Please make sure that you have a system with a turbomolecular high vacuum pump and not a diffusion pump as 2 ml/min exceeds the maximum allowable flow into a diffusion…
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Hi Margot,
It may well be that the column performance has deteriorated so much within the 7 months that the EasiVial standards are measured with poorer PDI values. The column performance can be tested with regular plate count determinations. It is a…
Hi sojeong,
every test report, delivered with aour columns, contains multiple specifications, but they are all measured with specialized systems and software. That means you might not be able to reproduce them identically. BTW for plate count you also…
Hi, I cannot configure the column compartment (CC) as it came integrated with the vial sampler on LC1290. We have the G7129B model for the integrated vial sampler and CC. I tried adding the column compartment details from the instrument configuration…
When the tuning is done at 3200 (+) everything is fine. When I change it to tune at 1700 (+) the masses are reasonably accurate. After it gets to the third mass (620) it appears that the entire spectrum shifts by about 1.5 AMU and the tuning fails. There…
Hi Siddhartha,
Thank you for contacting Cell Analysis Technical Support. Can you please go ahead and send us your System Files so that we can see what might be going on? These can be accessed in the Help Section of the Wave software. You can select…
This Information Applies To: Agilent 4200, 4150 and 2200 TapeStation.
Issue
Is it possible to analyze single stranded DNA (ssDNA) on the TapeStation? Which kit is the most suitable for analysis of ssDNA?
Resolution
Agilent does not offer any...
This Information Applies To: Agilent 4200 and 4150 TapeStation systems
Issue
How to Run the software installation verification on 4200 and 4150 TapeStation systems.
Background
The 4150 TapeStation model G2992A and 4200 TapeStation model G2991B...
This Information Applies To: Agilent 5200/5300/5400 Fragment Analyzer, and Femto Pulse
Issue
Perform a capillary alignment when a new array is installed in your Agilent Parallel Capillary Electrophoresis instrument. An alignment may also be performed...
This Information Applies To: Agilent 4200 and 4150 TapeStation
Issue
Are the Agilent DNA and RNA ScreenTape assays denaturing?
Resolution
The DNA ScreenTape assays in the TapeStation portfolio are not denaturing. Only the RNA and High Sensitivity...
This Information Applies To: Agilent LC pumps with manual purge valve.
Issue
A good practice before using a system is to purge the system to remove air. This is done by observing the clear tubing from the solvent bottle as well as the waste line...
This Information Applies To: ICP-MS MassHunter 4.x and 5.x
Issue
Unable to open a batch in offline data analysis, or the batch is empty.
If the ICP-MS MassHunter software closes unexpectedly, there is a power outage, or the ICP-MS and computer...
This Information Applies To: Agilent OpenLab CDS versions 2.4 or higher.
Issue
How to preview result files using various Report Templates in OpenLab CDS versions 2.4 or higher.
Requirements
The user has the result set loaded in OpenLab CDS...
This Information Applies To: ICP-MS MassHunter with Spectroscopy Database Administrator (SDA) and Protect Local Data ON.
Issue
An 'Authentication failed' error message appeared after entering user name and password when attempting to download a...
This Information Applies To: ICP-MS MassHunter with Spectroscopy Database Administrator (SDA) and Protect Local Data On.
Issue
With Spectroscopy Database Administrator (SDA) and Protect Local Data turned on, batches are not accessible through file...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
Scratches on AriaMx optical modules can lead to a reduction in their performance.
Background
Scratches on an AriaMx optical module are shown in Figure 1 .
Figure 1 ....