This Information Applies To: Agilent LC pumps with manual purge valve.
A good practice before using a system is to purge the system to remove air. This is done by observing the clear tubing from the solvent bottle as well as the waste line from the purge valve, until no air bubbles are noticed. There have been cases however, when the user will observe a continuous stream of air bubbles exiting the purge valve waste line even though no bubbles are observed in any of the lines from the solvent bottles.
The user assumes that the bubbles are coming from the pump and will continue to purge for some time without the bubbles being reduced. These bubbles can in fact be caused by air leaking into the waste tubing if the waste tubing is not fully seated and sealed onto the purge valve waste connection. This intake of air occurs due to the Venturi effect.
Warning: Take care when cutting the tubing to cut on a firm cutting surface and take care to avoid any injury from using a sharp cutting blade. Follow your organizations safety protocols and wear appropriate Personal Protective Equipment if necessary.
Learn how to effectively operate your Agilent 1260 Infinity LC system with OpenLab CDS:
Agilent 1260 Infinity LC system with OpenLab CDS e-learning path available from Agilent education