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  • Modified : 
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Automated Electrophoresis Systems
  • Component :  TapeStation Systems
  • Product Name :  2200 TapeStation System 4150 TapeStation System 4200 TapeStation System
  • Task :  Data Analysis
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Analysis of Single Stranded DNA on the TapeStation

Answer

This Information Applies To: Agilent 4200, 4150 and 2200 TapeStation. 

Issue

Is it possible to analyze single stranded DNA (ssDNA) on the TapeStation? Which kit is the most suitable for analysis of ssDNA?

Resolution 

Agilent does not offer any kit dedicated or validated for ssDNA analysis. None of the kits have been tested for performance with ssDNA.

However, given that the TapeStation RNA assays are validated for total RNA, which is a single stranded nucleic acid, the RNA kits can be considered. The dye used in the RNA kits has a higher affinity for single stranded material than the dye used in the DNA kits. 

The migration profile, sizing, and quantification might not be accurate for ssDNA because the RNA kits were validated for total RNA samples. Agilent cannot guarantee that data obtained for ssDNA using RNA kits is within the given specifications of the respective assay. 

  
Caution: Agilent does not support and has not validated the examination of ssDNA samples on the TapeStation. 

 

  
Learn how to effectively operate and maintain your Agilent TapeStation:
Agilent 4200 TapeStation System Manual on Agilent.com

For Research Use Only. Not for use in diagnostic procedures.

PR7001-1516

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