This Information Applies To: Agilent 5200/5300/5400 Fragment Analyzer, and Femto Pulse
Perform a capillary alignment when a new array is installed in your Agilent Parallel Capillary Electrophoresis instrument. An alignment may also be performed during troubleshooting.
Steps to Follow
Tip: An optical alignment can only be performed without dye for a 12- or 48-capillary array.
Tips: Avoid the Top Red CCD Camera Reference Area and the Capillary Alignment References.
Figure 3. Capillary alignment display. This screenshot is from a 5200 Fragment Analyzer.
1. Contrast slider. 2. CCD Camera reference. 3. Box drawn by left-clicking and dragging. 4. Capillary alignment references.
|Align||Aligns cursors to peaks.|
|Save||Saves changes to the alignment and exits the window.|
|Cancel||Cancels any actions and closes the file.|
|Auto Space||Auto locates the capillary positions based off the first capillary position. Positions will need manual adjustment.|
|Read raw||Opens the Align from File window allowing the user to complete the capillary alignment using a previously run file.|
Table 1. Capillary alignment display menu options.
Learn how to effectively maintain and operate your Agilent Parallel Capillary Electrophoresis systems:
5200, 5300, and 5400 Fragment Analyzer System Manual on Agilent.com
Femto Pulse User Manual on Agilent.com
For Research Use Only. Not for use in diagnostic procedures.