• Created : 
  • Modified : 
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Automated Electrophoresis Systems
  • Component :  ScreenTapes and Reagents TapeStation Systems
  • Product Name :  2200 TapeStation System 4150 TapeStation System 4200 TapeStation System Cell-free DNA ScreenTape Analysis DNA ScreenTape Analysis Genomic DNA ScreenTape Analysis High Sensitivity DNA ScreenTape Analysis High Sensitivity RNA ScreenTape Analysis RNA ScreenTape Analysis
  • Task :  Operation
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Are TapeStation Assays Denaturing?

Answer

This Information Applies To: Agilent 4200 and 4150 TapeStation

Issue

Are the Agilent DNA and RNA ScreenTape assays denaturing? 

Resolution 

The DNA ScreenTape assays in the TapeStation portfolio are not denaturing. Only the RNA and High Sensitivity RNA ScreenTape assays are denaturing.

 Components in the RNA and High Sensitivity RNA sample buffer partially denature the sample. There is also a heat denaturation step included in the RNA and High Sensitivity RNA operating procedure in the assay Quick Guides.

 
Learn how to effectively operate and maintain your Agilent TapeStation: 
Agilent 4200 TapeStation System Manual on Agilent.com
Agilent 4150 TapeStation System Manual on Agilent.com

For Research Use Only. Not for use in diagnostic procedures.

PR7001-1670

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