Using the Agilent 7890A. Column installed with no leaks detected. Both the front and back detector are set to 300°C, gas Flow Rates - Hydrogen: 45mL/min, Air 450mL/min and He 1.0mL/min. The split flow is 80mL/min. Following initial start up the front…
Thanks for the tips on seeing the position in the sequence once Aquisition has been restarted. Unfortunately something I failed to mention is that the sequence also disappears after pressing run but will re-appear once you toggle between another module…
Hi vitiin96,
this is very generic and any book on GC troubleshooting or the videos on the Agilent website could help. For the 7890 there is a troubleshooting manual available.
Good practice in GC is when you get a (new) column, you have an own suitability…
I have started using GC-FID (Agilent 8890) since March for the first time. We are trying to measure the methane. I was trying to make a standard surve, so I injected 1000 ppm methane standard at first. Just to let you guys know no samples has been run…
no you don't need to disconnect it from the FID, ,
bake the column for enough time usually more than 1 hour according to your column status , you can keep the FID working to monitor the baseline .
..
keep temperature 25 degrees less than the maximum…
Get a WS asynchronous error : F F60304 The torch ignition has failed when starting a Varian 720 OES The torch lights and stays on for about 5 seconds then shuts down with this error. I can not find reference in the help to resolve this. Thanks, Scott
Hi all, I am using a GC 6890 with split/splitless inlet to analyse reaction products. However, a problem has recently happened when the GC is running. I have noticed the appearance of spikes in the baseline of the FID detector. The spikes appear occasionally…
It looks like it is having to clear something out the inlet, column, detector or head space as the baseline rise is over most of it. I see it's a FID you are using, how long do you leave it ignited before running the first analysis?
Hi John, You are on the right track-- it sounds like your flame is not lit. The reading you are seeing on the Hydrogen line is likely back-pressure from the column flow. That result could indicate a blockage in the jet, but the flow is a little low to…
Hello, im having a problem with a 2d GC using the Deans Switch system. Its a 8890 GC with 2 FIDs. The problem is that, when cutting peaks from the 1st dimension to the 2nd dimension (2nd column), all peaks on the 2nd dimensions get split into 2 peaks…
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Issue
The Agilent 2100 Bioanalyzer system run ends prematurely and the following error appears in the logbook: Run aborted on port x. Instrument error occurred on port x, Unusual...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Issue
The Agilent 2100 Bioanalyzer system electropherograms in the Agilent 2100 Expert software have a wavy, uneven baseline ( Figure 1 ).
Figure 1. Wavy, uneven baseline on Bioanalyzer...
This Information Applies To: Agilent AriaMx systems.
Issue
When IP addresses of an AriaMx instrument are reassigned, it will appear under the new and the previous IP address in the AriaMx Instrument Explorer. This situation can be prevented using...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
Sometimes, for support purposes, it is necessary to send the diagnostic test result file to Agilent support representatives.
This file contains detailed information about the...
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx Real-Time PCR software
Issue
How to change the discovery protocol port in the AriaMx software.
Steps to Follow
The AriaMx software uses the UDP Broadcast protocol for...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
The instrument diagnostic tests should be run whenever it is suspected that there could be a fault with the instrument hardware.
Background
The instrument diagnostics consist...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
Error 45 is displayed on the AriaMx instrument touch screen.
Background
Error 45 is shown when the temperature recorded by one or more thermistors is outside the expected...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
AriaMx showing a series of errors after delivery, guide for troubleshooting and resolving temperature-related error.
Background
When an AriaMx is delivered, it could take...
How to Access Manual Tune in GC/MS MassHunter and MSD Productivity ChemStation
Multiple Results
This Information Applies To: MassHunter acquisition software for GC/MS systems and MSD Productivity ChemStation for all Agilent GC/MS systems
Issue
Manual tuning lets you interactively set the MSD parameters, such as lens voltages and tuning masses...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
How to find the AriaMx software and firmware version that you have installed.
Steps to Follow
There are two different ways to find out AriaMx software version:
In the...