This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
The Agilent 2100 Bioanalyzer system electropherograms in the Agilent 2100 Expert software have a wavy, uneven baseline (Figure 1).
The error is most often associated with:
Steps to Follow
To prevent the unnecessary consumption of multiple chips during troubleshooting, please read this article in full and perform all recommended maintenance before running another chip.
To exclude a problem with the chip priming
To exclude a dirty or wet electrode cartridge
Caution: Damp pin set: Make sure that the pin set is fully dry before placing it back into the electrode base. Even small amounts of liquid on the pin set can damage the high-voltage power supply. Before starting a run, check that the pin set is dry by running the short circuit test as described in chapter 4 of the Maintenance and Troubleshooting guide.
To exclude a problem with the chip preparation
To exclude a problem with the samples
The wavy baseline symptom can be sample-related. Chip overloading and contamination with high molecular weight molecules can cause a wavy baseline.
To exclude a problem with the instrument
Learn how to effectively maintain your Bioanalyzer: