This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
The Agilent 2100 Bioanalyzer system run ends prematurely and the following error appears in the logbook: Run aborted on port x. Instrument error occurred on port x, Unusual high or low voltage or current was detected during the start phase of the on-chip analysis. The marked wells will indicate the wells on the chip that caused the problem. An example of the error is shown in Figure 1.
The error is most often associated with insufficient volume in one or more of the chip wells, problems during the priming, presence of bubbles in the gel matrix, dirty electrodes, or moisture on the electrodes.
To prevent the unnecessary consumption of multiple chips during troubleshooting, please read this article in full and perform all recommended maintenance before running another chip.
To exclude a problem with the liquid in the wells
To exclude a problem with the chip priming
To exclude a dirty electrode cartridge
Caution: Damp pin set: Make sure that the pin set is fully dry before placing it back into the electrode base. Even small amounts of liquid on the pin set can damage the high-voltage power supply.
To exclude a problem with the instrument
Learn how to effectively maintain and operate your Agilent Bioanalyzer:
For Research Use Only. Not for use in diagnostic procedures.