• Created : 
  • Modified : 
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Real-Time PCR (qPCR)
  • Component :  qPCR Systems
  • Product Name :  AriaMx Real-time PCR System
  • Task :  Operation
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Error 45 on the AriaMx

Answer

This Information Applies To: Agilent AriaMx real-time PCR system

Issue

Error 45 is displayed on the AriaMx instrument touch screen. 

Background

Error 45 is shown when the temperature recorded by one or more thermistors is outside the expected range. The operating temperature range for the AriaMx is 20 to 30 °C.

Steps to Follow

  1. Make sure that the instrument is installed in a room within the operating environment temperature range (20 to 30 °C). If the temperature of the room is too hot or cold, adjust the room temperature to within the required range. If you would prefer to move the AriaMx to a different location that is within the correct temperature range.

      
    Tip: The performance of the AriaMx could be adversely affected by bumping it hard or shaking it vigorously. Moving the instrument between labs (on the same floor) can be handled on a sturdy cart.  For longer moves, contact your local Agilent sales and support center for assistance in moving the Aria and assuring proper operation afterwards.

  2. The temperature sensors can be sensitive to environmental conditions, so make sure that the instrument is not in direct sunlight, by an open window, near a heater or an air conditioning vent.

  3. Check the block temperature shown in the top left of touch screen of the AriaMx (Figure 1) to verify that the instrument is operating within the indicated temperature range.


    Block Temperature.
    Figure 1. AriaMx touch screen with the block temperature in the top-left corner highlighted with a yellow square.

  4. Run a diagnostic check.
   Tip: To run the diagnostic check, log in as an administrator account (the default password for the Administrator user name is ADMIN). On the instrument touch screen, select Settings > Instrument Diagnostics. Deselect the observational test section as these tests are not required to remove error 45. Leave all other tests selected. Select Run. This is also described in chapter 2 of the AriaMx Real-Time PCR System Setup and User Guide.

Root cause

  • Environmental temperature is outside recommended range
  • Hardware failure

 

 

Learn how to effectively operate your Agilent AriaMx Real-Time PCR System:
AriaMx Real-Time PCR System Setup and User Guide on Agilent.com. 
Aria Real-Time PCR Software User Manual on Agilent.com.

For Research Use Only. Not for use in diagnostic procedures.

PR7001-1268

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