Hello
I need to do an LC/MSMS method for quantification on a compound that doesn't ionize into [M+H]+ (barely observed) but only into [M-H2O+H]+
Is there a problem with doing MS/MS on a [M-H2O+H]+ parent ion ?
(PS: negative ionization is not an…
Hi again Raina,
as I already told you this cannot be done with just one column, especially with the CO2 concentration up to 20% and water included and the necessity to analyze H2. This is an application we normally build an analyzer for. You need at…
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Operating like this puts sub-optimal flow into the GCMS ion source of more than 2 ml/min. Please make sure that you have a system with a turbomolecular high vacuum pump and not a diffusion pump as 2 ml/min exceeds the maximum allowable flow into a diffusion…
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Hi Margot,
It may well be that the column performance has deteriorated so much within the 7 months that the EasiVial standards are measured with poorer PDI values. The column performance can be tested with regular plate count determinations. It is a…
Hi sojeong,
every test report, delivered with aour columns, contains multiple specifications, but they are all measured with specialized systems and software. That means you might not be able to reproduce them identically. BTW for plate count you also…
Hi, I cannot configure the column compartment (CC) as it came integrated with the vial sampler on LC1290. We have the G7129B model for the integrated vial sampler and CC. I tried adding the column compartment details from the instrument configuration…
When the tuning is done at 3200 (+) everything is fine. When I change it to tune at 1700 (+) the masses are reasonably accurate. After it gets to the third mass (620) it appears that the entire spectrum shifts by about 1.5 AMU and the tuning fails. There…
Hi Siddhartha,
Thank you for contacting Cell Analysis Technical Support. Can you please go ahead and send us your System Files so that we can see what might be going on? These can be accessed in the Help Section of the Wave software. You can select…
This Information Applies To: Agilent 4200 TapeStation 4150 TapeStation, TapeStation controller software
Issue
The system verification can be run to check the TapeStation performance, for example, when you suspect an instrument hardware fault.
...
This Information Applies To: Agilent 4200 and 4150 TapeStation software.
Issue
How to uninstall and reinstall the 4200 and 4150 TapeStation software.
Background
An uninstallation and reinstallation of the TapeStation software should be performed...
This Information Applies To: 2200 TapeStation instruments, 2200 TapeStation controller software
Issue:
The Agilent 2200 TapeStation controller software won't launch but displays the correct serial number in the top-right corner of the loading screen...
This Information Applies To: 4200, 4150 and 2200 TapeStation, TapeStation analysis software A02.02 or higher.
Issue:
When using the Agilent 4200, 4150 and 2200 TapeStation systems, the DV 200 of FFPE RNA samples can be calculated within the TapeStation...
This Information Applies To: 5200/5300/5400 Fragment Analyzer, Femto Pulse, ZAG DNA Analyzer, ProSize Data Analysis Software
Issue
How to export a ladder as a size calibration file and import it into a different run in ProSize.
Background
...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue
It is possible to set up the 2100 Expert software so that the files are automatically exported each time a chip run is complete.
Steps to Follow
To turn...
This Information Applies To: 2100 Bioanalyzer system, 2100 Expert software
Issue:
How to identify and troubleshoot late migration of the ladder, marker, and sample peaks in data generated on the Agilent 2100 Bioanalyzer system.
Background
...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue
If communication between the 2100 Bioanalyzer and the PC cannot be established, or if an intermittent communication loss occurs, follow the instructions in...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue
Instructions on how to import from or export data into LIMS software.
Steps to Follow
Data import
When importing sample names and sample comments from...