This Information Applies To: 4200, 4150 and 2200 TapeStation, TapeStation analysis software A02.02 or higher.
When using the Agilent 4200, 4150 and 2200 TapeStation systems, the DV200 of FFPE RNA samples can be calculated within the TapeStation analysis software. The DV200 score is a quality score for evaluating quality of RNA derived from formalin-fixed paraffin-embedded (FFPE) samples established by Illumina Inc. in 2016. It represents the percentage of RNA fragments larger than 200 nucleotides relative to all RNA fragments.
Steps to follow
The workflow to calculate the DV200 described here applies to software version A.02.02 or higher. If you are using an earlier version, download the 2200 TapeStation software or 4200/4150 TapeStation software. To apply the DV200 calculation to an existing data file (Figure 1):
Once the DV200 region is defined, the value is provided in the region table in the % of total column for each sample.
See the Technical Overview for more details.
Figure 1. How to add a DV200 region in the TapeStation analysis software
Learn how to effectively maintain and operate your TapeStation: