This Information Applies To: 5200/5300/5400 Fragment Analyzer, Femto Pulse, ZAG DNA Analyzer, ProSize Data Analysis Software
How to export a ladder as a size calibration file and import it into a different run in ProSize.
In the Agilent ProSize analysis software, you can calibrate the size of sample fragments in two ways. You can, either by running a standard DNA or RNA Ladder in a well of the sample plate (recommended), or by import a previously calculated and exported size calibration file (*.scal extension).
Caution: When using an imported size calibration, the separation gel, capillary array dimensions, injection/run voltage, and markers should be identical for the analyzed data and imported calibration to maximize sizing accuracy.
It is important to ensure the sample containing the size calibration ladder has all ladder fragments integrated correctly with no additional peaks before analyzing the remaining samples.
Differences between the analyzed and imported conditions will adversely affect the sizing accuracy. It is recommended to only use a .scal file for one week after creation for accurate results. The most accurate data can only be gathered by running a ladder with each set of samples. (A caution message advise readers that failure to take or avoid a specific action could result in damage to equipment or loss of data.)
Steps to Follow
To export a .scal file, carry out the following steps, which are also shown in Figure 1.
Figure 1. Exporting a .scal ladder file
To import a .scal file, carry out the following steps. These steps are shown in Figure 2.
Figure 2. Importing a .scal ladder file
Learn how to effectively maintain and operate your parallel capillary electrophoresis instrument:
5200, 5300, and 5400 Fragment Analyzer System Manual on Agilent.com
Femto Pulse user manual on Agilent.com
ZAG DNA Analyzer system manual on Agilent.com
For Research Use Only. Not for use in diagnostic procedures.