The file is a pre-set qPCR program to run a plate filled with water used to test the basic raw fluorescence signal of AriaMx Optical modules.
File required for Performing a Background Calibration
The GC cycle time should be the time from injection until the GC is ready to run again. This includes the analysis run, cool down, and equilibration. Based on the times you listed, it would be roughly 40 minutes. It may be worth timing to make sure. The…
Hi howard_sanford, my apologies for a slow response, but thank you very much for the help and advice. I was able to get the filter for Cal and CC to work, and I appreciate the discussion of my larger problem. I think I'm probably several (many) steps…
Hello, thank you for your help. I had tried loading different processing methods but I didn't realize the results were not linked to any method. It turns out the result files were read-only and could not be linked to any method. I moved the result files…
The peaks look similar, essentially the same sensitivity. I'd look at the background on your older runs and the background now. It could be that most of the previous background was one, two, a few, ions and they're gone now. Was that small but still noticeable…
That is one failure mode of Platinum Resistance Thermometers. It can happen one degree to the next higher degree. The heater/sensor must be replaced, part number G1099-60107.
See also: (+) AUX 2 heater fault - Forum - GC/MS - Agilent Community
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
AriaMx touch screen displays error code 90 after the Instrument Health Check (IHC), which happens when the AriaMx is switched on, or after running the onboard diagnostic tests...
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx optical modules
Issue
AriaMx users can change which AriaMx optical modules are installed to upgrade the instrument capabilities or to troubleshoot.
Items Required
Any...
This Information Applies To: AriaMx real-time PCR system
Issue
Error 43 is displayed on the AriaMx touch screen. Error 43 is a voltage error and it normally indicates that the cartridge LED voltage or motor voltage being detected is abnormal. Sometimes...
This Information Applies To: Agilent 2100 Bioanalyzer system
Issue
Can I run qPCR samples containing Sybr® Green I on the Bioanalyzer?
Background
Sybr® Green I is an Intercalating Dye commonly used to detect amplification products in real...
This Information Applies To: Agilent 4200, 4150 and 2200 TapeStation.
Issue
Is it possible to analyze single stranded DNA (ssDNA) on the TapeStation? Which kit is the most suitable for analysis of ssDNA?
Resolution
Agilent does not offer any...
This Information Applies To: Agilent 4200 and 4150 TapeStation systems
Issue
How to Run the software installation verification on 4200 and 4150 TapeStation systems.
Background
The 4150 TapeStation model G2992A and 4200 TapeStation model G2991B...
This Information Applies To: Agilent 5200/5300/5400 Fragment Analyzer, and Femto Pulse
Issue
Perform a capillary alignment when a new array is installed in your Agilent Parallel Capillary Electrophoresis instrument. An alignment may also be performed...
This Information Applies To: Agilent 4200 and 4150 TapeStation
Issue
Are the Agilent DNA and RNA ScreenTape assays denaturing?
Resolution
The DNA ScreenTape assays in the TapeStation portfolio are not denaturing. Only the RNA and High Sensitivity...
This Information Applies To: Agilent LC pumps with manual purge valve.
Issue
A good practice before using a system is to purge the system to remove air. This is done by observing the clear tubing from the solvent bottle as well as the waste line...
This Information Applies To: ICP-MS MassHunter 4.x and 5.x
Issue
Unable to open a batch in offline data analysis, or the batch is empty.
If the ICP-MS MassHunter software closes unexpectedly, there is a power outage, or the ICP-MS and computer...