This Information Applies To: Agilent 2100 Bioanalyzer system
Can I run qPCR samples containing Sybr® Green I on the Bioanalyzer?
Sybr® Green I is an Intercalating Dye commonly used to detect amplification products in real-time PCR. This dye interacts with single and double stranded DNA in a nonspecific manner. It shows an increased fluorescence intensity upon binding to DNA.
It is possible to run and detect qPCR samples on the Bioanalyzer even in the presence of Sybr® Green I. Amplicons containing Sybr® Green I can be detected without disturbances using the DNA 1000 assay on the Bioanalyzer.
Caution: The incorporation of Sybr® Green I into the sample results in a small but significant increase of fluorescence intensity and a small but significant increase in the electrophoretic mobility. These changes must be considered when running samples containing Sybr® Green I on the Bioanalyzer.
Learn how to effectively maintain and operate your Agilent 2100 Bioanalyzer system:
2100 Expert Software User's Guide on Agilent.com
Agilent 2100 Bioanalyzer System Maintenance and Troubleshooting Guide on Agilent.com
For Research Use Only. Not for use in diagnostic procedures.