Analyze Agilent Seahorse XF Real-Time ATP rate assay data using Agilent Seahorse Analytics
Analyze Agilent Seahorse XF Hu T Cell Activation Assay data using Agilent Seahorse Analytics software
Analyze Agilent Seahorse XF Substrate Oxidation Stress…
Hello APacana ,
The reason for the difference in response between the TIC and the EIC is because when you extract the TIC of a given precursor you are extracting the ion signal from all precursors that create a 72 product ion, using your example. If…
Hello AJohnson13 ,
Here is the PC setup guide for older Windows 10 versions of MassHunter. It includes the PC specifications.
Windows10_Setup.book (agilent.com)
The software installation instructions would be included as part of your original media…
At 1 mL/min pumping water, the pressures are as follows:
Pump + Column = 53 bar
Pump + Column + 0.1 mm ID 30 cm tubing = 70 bar
Pump + Column + Tubing + Flow Cell = 75 bar
I have also confirmed that this pressure fluctuation has nothing to do…
It all depends on your following steps. You can even use Methanol or Acetonitrile for the protein precipition: Protein Precipitation for Biological Fluid Samples Using Captiva EMR-Lipid
See also the chapter on protein precipitation in Agilent's Handbook…
The Digest of upcoming webinars has been updated as of April 30.
Did you miss April's GC Live Session Recording and Discussion Thread? Check it out here: ( +) Diagnostics From Your Desk, Troubleshooting From Your Kitchen Table - Follow-up Discussion…
Hello cjwwfd ,
B.06.00 is out of support at this point, but I can offer some general suggestions for current revisions and hopefully you can apply them to your version. Version 12.0 is the current release. If possible, I would investigate your options…
I'm experimenting the same error. Logbook displays <MSQQQ_1: QqQ fault detected: 1.23 vacuum system is not ready. This is typical during a pump down cycle or when the high vacuum pump is turned off during a vent cycle or fault condition>. Does anyone…
Hello DET ,
Sorry for the late response on this.
What sort of spiking do you see usually? What is the output of the FID, in pA?
Are you using any UPS systems, or are the GCs plugged directly into the wall?
This Information Applies To: Fragment Analyzer, Femto Pulse, ZAG, and their respective controller software
Issue:
If there is dried gel in the reservoir vent valve of your Agilent Fragment Analyzer, Femto Pulse, or ZAG, cleaning is required. A clogged...
This Information Applies To: 5200, 5300, 5400 Fragment Analyzer, Femto Pulse, Oligo Pro II, and ZAG
Issue:
Adding mineral oil to Agilent parallel capillary electrophoresis DNA marker plates and samples plates prevents evaporation and enables re...
This Information Applies To: 5200/5300/5400 Fragment Analyzer, Femto Pulse, Oligo Pro II, and ZAG
Issue:
Only plasticware from the approved consumables list should be used in the Agilent Parallel Capillary Electrophoresis systems. The use of plasticware...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Question:
Can more than one Agilent 2100 Bioanalyzer instrument be connected to one PC?
Steps to follow:
It is possible to connect a maximum of two Bioanalyzers to one laptop...
This Information Applies To: 2100 Bioanalyzer, Bioanalyzer Chip Priming Station: part number 5065–4401
Maintenance procedures and how frequently they are required :
Procedure
Time interval
Or if this issue occurs
Where to find a detailed...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Issue:
Running the hardware diagnostics on the Agilent 2100 Bioanalyzer system can help to investigate whether the instrument performs as expected.
Steps to follow:
Required...
This Information Applies To: 2100 Bioanalyzer, RNA 6000 Nano kit, RNA 6000 Pico kit, Small RNA kit
Issue:
Is it possible to size RNA on the Agilent 2100 Bioanalyzer system?
Resolution:
Agilent offers total and small RNA assays on the Bioanalyzer...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue:
The Agilent 2100 Bioanalyzer system does not detect a prepared microfluidic chip after it is inserted into the instrument. The interface shows the instrument...
This Information Applies To: 2100 Bioanalyzer, Bioanalyzer chips
Issue:
Can 2100 Bioanalyzer assay chips and reagents be interchanged?
Resolution:
Some assay chips can be used with multiple assays, however some cannot because the chip layout...
This Information Applies To: Agilent MassHunter GC/MS Acquisition software
Issue
The MassHunter software performs several checks during the startup sequence. If it detects a problem, an alert message box will be displayed and the message bar on...