Best precipitation reagent

Question

Hi Community 
 With regards to precipitation proteins from biological matrices like serum and plasma, which acid is more preferrable, HCL or Sulphuric acid? 
 Regards 
 BSouth

Dr. Know · Accepted Answer

It all depends on your following steps. You can even use Methanol or Acetonitrile for the protein precipition: Protein Precipitation for Biological Fluid Samples Using Captiva EMR-Lipid 
 See also the chapter on protein precipitation in Agilent's Handbook of Sample Preparation: Sample Preparation Fundamentals 
 A doctoral thesis from the Technical University of Delft looks into the mineral acids.

Best precipitation reagent

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