I can't seem to get our new instrument to pass an EPA DFTPP tune for method 525.2. We recently got an instrument with the hydroinert source and I have only gotten a couple of passing tunes but nothing that is repeatable. I have tried manual tuning myself…
Yes! I checked the line too as according to the instructions (+) How to Disassemble and Assemble the HES (High Efficiency Source) - Articles - GC/MS Portal - Agilent Community
I will take a picture when I have them out next time. Thank you so much for…
Analyze Agilent Seahorse XF Real-Time ATP rate assay data using Agilent Seahorse Analytics
Analyze Agilent Seahorse XF Hu T Cell Activation Assay data using Agilent Seahorse Analytics software
Analyze Agilent Seahorse XF Substrate Oxidation Stress…
Hello APacana ,
The reason for the difference in response between the TIC and the EIC is because when you extract the TIC of a given precursor you are extracting the ion signal from all precursors that create a 72 product ion, using your example. If…
Hello AJohnson13 ,
Here is the PC setup guide for older Windows 10 versions of MassHunter. It includes the PC specifications.
Windows10_Setup.book (agilent.com)
The software installation instructions would be included as part of your original media…
At 1 mL/min pumping water, the pressures are as follows:
Pump + Column = 53 bar
Pump + Column + 0.1 mm ID 30 cm tubing = 70 bar
Pump + Column + Tubing + Flow Cell = 75 bar
I have also confirmed that this pressure fluctuation has nothing to do…
It all depends on your following steps. You can even use Methanol or Acetonitrile for the protein precipition: Protein Precipitation for Biological Fluid Samples Using Captiva EMR-Lipid
See also the chapter on protein precipitation in Agilent's Handbook…
The Digest of upcoming webinars has been updated as of April 30.
Did you miss April's GC Live Session Recording and Discussion Thread? Check it out here: ( +) Diagnostics From Your Desk, Troubleshooting From Your Kitchen Table - Follow-up Discussion…
This Information Applies To: 4150 TapeStation, 4200 TapeStation
Issue
How to deal with a TapeStation Electrode Cartridge change is due warning message.
Background
The Agilent TapeStation controller software will notify users to replace the...
This Information Applies To: Agilent OpenLab CDS
Issue
Sometimes the integration result is not ideal for all injections. This article outlines the step-by-step instruction to manually integrate peaks.
Background
There are 2 ways to integrate...
This Information Applies To: Agilent OpenLab CDS , Agilent OpenLab ChemStation
Issue
Calculation of Area percent or Height percent for uncalibrated compounds in OpenLab software. If all components respond equally in the detector, then Area% and...
This Information Applies To: Agilent ICP-MS (7700/7800/7850/7900/8800/8900)
Issue
How to power off the Agilent ICP-MS Foreline Pump to perform maintenance, or when fully powering off the Agilent ICP-MS System.
Background
The ICP-MS MassHunter...
This Information Applies To: Agilent ICP-MS (7500,7700,8800)
Issue
How to check the E2M18 or E2M18F foreline pump to determine if the pump needs maintenance?
Background
The oil cleanliness and oil level in the E2M18 or E2M18F foreline pump...
This Information Applies To: Agilent ICP-MS (7700/7800/8800)
Issue
How to replace the foreline pump oil in the DS 402.
Background
The DS 402 oil should be replaced at least every 3 months or when the oil color indicates it needs to be changed...
This Information Applies To: Agilent 8890/8860 GC Systems
Issue
How to delete data that is stored on the GC instrument.
Steps to follow
Caution : Before deleting data from your GC, Agilent recommends extraction of data as a backup...
This Information Applies To: 5200, 5300 and 5400 Fragment Analyzer, Femto Pulse and ZAG DNA analyzer
Issue
When no data is available in the Agilent ProSize data analysis software for one or more capillaries, the capillary(ies) could be misaligned...
This Information Applies To: Agilent ICP-MS (7800/7850/7900/8900)
Issue
How to check the MS40+ or MS40-S foreline pump to determine if the pump needs maintenance?
Background
The oil's cleanliness and level, in the MS40+ or MS40-S foreline...
This Information Applies To: Agilent ICP-MS systems (7700/7800/7850/7900/8800/8900)
Issue
Powering on Agilent ICP-MS Systems after maintenance, long-term shutdown, or for power cycling.
Steps to Follow
Confirm that the ICP-MS power plug...