How to Do Manual Integration in OpenLab CDS

Answer

This Information Applies To: Agilent OpenLab CDS

Issue

Sometimes the integration result is not ideal for all injections. This article outlines the step-by-step instruction to manually integrate peaks.

Background

There are 2 ways to integrate peaks in OpenLab CDS:

1. Edit integration events 
2. Manually integrate peaks 

With the Manual Integration tools in Data Analysis, you do not need any toolbar buttons to choose the right tool. The system automatically selects the appropriate tool when you move the mouse pointer over the chromatogram. Visual feedback is continuously given to indicate what tool is active and what part of the data will be modified by the currently active tool.

Figure 1 shows an example of the Intelligent Cursor in action. A meaningful command hint (Split Peaks in this case) and a specific cursor indicate the active tool. The affected peaks are highlighted.

Figure 1. Example of Curosr

Many of the Manual Integration tools interact with Attractors . They are usually visible on the signal and at baseline points of the adjacent elements. When the Cursor approaches an Attractor, it will automatically snap to the Attractor. This allows you to position new elements directly on the signal or directly attached to adjacent baseline segments (see Figure 2)

Figure 2. Example of Attractor  

Prerequisite

• To be able to carry out the procedure as described, you need the privileges Data Processing > Do manual integration and Processing Method > Edit integration parameters. Privileges are configured in the Control Panel.
• If the project enforces method approval, the status of the result set method that you want to update must be Generic. For details, see Method Approvals.

Steps to Follow

1. Make sure there is a method linked to your data. See Link injections to a processing method.
2. Display the chromatogram, and zoom into the area of interest.
3. Click the Manual Integration icon in the Chromatograms toolbar (Figure 3).
   

   
   Figure 3. Manual integration icon
   

4. Optional: To adjust the settings for manual integration, click the Properties icon in the Chromatograms toolbar (Figure 4). For more details, see Manual Integration Options and Tools.
   

   
   Figure 4. Properties icon 
   

5. Move the mouse cursor over the signal or the area of the baseline where you want to change the integration.
   Depending on the position of the mouse cursor, the system will offer the relevant manual integration tool.
6. When the required manual integration tool is active, drag the mouse to the new position, or click at the desired position.
   The injection is automatically reprocessed. An asterisk (*) is appended to the filename in the injection tree to indicate the manual integration and the Baseline Code is m.
   As the changed integration will change the results, any existing signatures will not be carried forward. A dialog will ask for confirmation.
7. To undo or redo a manual integration correction, click the Undo  or Redo icon in the Chromatograms toolbar.
8. To build a calibration curve with the adjusted integration values, reprocess the entire sequence. Click Reprocess All to reprocess the entire sequence.
9. To save manual integration corrections in the processing method, click in the Chromatograms toolbar. For details, see Apply manual integration corrections to other injections.

Caution: Manually integrated peaks are deleted if you click - Reprocess All - Clear corrections.

Figure 5 shows a video how to do manual integration in OpenLab CDS 2.6.

Figure 5. Manual Integration in OpenLab CDS 2.6 

Learn how to effectively operate your Agilent OpenLab CDS Software: HPLC-OLII-1140eV2 - Agilent Infinity LC systems with OpenLab CDS - Integration and viewing results (Ver. 2.6) e-learning course available from Agilent Education