Hi everyone I have a strange issue to report. We are using a 6890GC with FID and TCD that was upgraded many years ago to give use LAN communication rather than GPIB. It works great even though it is over 20 years old. Just prior to Christmas we installed…
Hi,
1. It is not normal, that you have to purge the cell gas lines every time
2. Ou should perform at least following "Tests" during Plasma ignition: Torch Axis - EM - Resolution/Axis. But a performance report every day would be nice (and therefore…
Varian 710 oes, nebulization is ok, fog in the chamber is omogeneous, tubing, nebulizer, torch, cone cleaned and alligned.
Work fine till 2 weeks ago, after i've experienced this: fail to execute wavelenght calibration end torch allign, No response…
Hello Everyone,
I am using 7820A GC coupled with 5977B Mass Spec. After use I close the He tank and later on I get 2 messages - 'Front inlet flow shutdown' and 'Front inlet pressure shutdown'. I restarted the software but it fails to load any GC method…
I am building a report for some GC-FID data from a GC-MS/FID instrument. I have managed to build a report that contains the chromatogram and the table of peaks. I can scale the X-axis to the scale i need but I have been unable to scale the y-axis to show…
I am using OpenLab Chemstation C.01.10 on an 8890 with dual FIDs. I was trained on HP's version of Chemstation that reported both detector quant amounts in one row for precision purposes. It made it 10x easier to review data and confirm sample concentrations…
Hello, we are setting up a seahorse assay for a immunology master class. We want to measure the glycolytic rate in human PBMCs or purified human CD4 T cells activated vs non activated. We fail to see any difference. Has anyone done this kind of assay…
We have a Duo 2.5 foreline pump that was replaced in June of this year (2023). The previous Duo 2.5 installed had a sudden catastrophic leak, thus the replacement in June. The oil was filled up halfway and I set a marker with tape to monitor the level…
Hi Juliana
Yes, most likely the capacitors and/or the RF Tube. However, you can try to reduce the run power to 1.1Kw in the default ignition table. You may then be able to run for a few weeks with this reduced power level. You will need to reduce the…
Hi byoo,
Do you get any failed to communicate or any other message when you start up the software? You are correct if the busy light should be constantly flashing. This means you got a successfuk firmware load from the PC from ethernet connection. Can…
This Information Applies To: HES ion source in 5977B/C GC/MSD, 7010 triple quadrupole GC/MS systems
Issue
The filaments are replaced as part of routine maintenance, or when they break (zero output current).
Items Required
Gloves, nylon,...
This Information Applies To: Agilent 5977A/B/C GC/MSD
Issue
The Electron Multiplier Horn must be replaced when it is contaminated or the EMV value reaches 3000 V with a clean ion source.
Items Required
Electron multiplier horn (5977B/C:...
This Information Applies To: Agilent 5973/ 5975/5977 GC/MSD with rotary vane foreline pump
Issue:
The oil mist filter is recommended to be replaced annually. If the color of the filter has changed or there is smell from the filter, the oil mist...
This Information Applies To: Agilent EI Source
Issue:
During normal operation of the GC/MS, the EI SST/Inert ion source will need to be disassembled to proceed with abrasive ion source cleaning and / or routine replacement of consumable parts (filaments...
This Information Applies To: Agilent G7129A/B/C Vialsampler
Issue:
Replacing the needle and needle seat of the Vialsampler is required when:
The needle is visibly damaged.
Leaks or blockages in the vialsampler are observed, especially between...
This Information Applies To: Agilent G1311A/B/C, G7111A/B, Quaternary Pumps, and G1310A/B, G7110B Isocratic Pumps, G1312A/B/C, G7112B Binary Pumps
Issue:
How to diagnose no flow or no pressure issues on binary, quaternary, or isocratic pumps.
...
This Information Applies To: Agilent UV and RID detectors
Issue
A clogged flow cell causes high backpressure. The clog can be removed by flushing the system with solvents at high pressure. If you suspect a clog or experience high backpressure due...
This Information Applies To: Agilent G1312X and G7112X binary pumps.
Issue:
A blockage before the pressure sensor, e.g. in a pump head, will not give a high pressure or overpressure system error. Instead, the pump may show one of various error messages...
This Information Applies To: Agilent 1100, 1200, 1260 LC Pumps.
Issue:
The following procedure allows the cleaning of residue from the pump's check valves, often caused by polymerization of aged acetonitrile or salt-based solutions that may prevent...
This Information Applies To: Agilent LC Systems
Issue
After analyzing dirty or concentrated samples, some sample compounds may retain in the column and other parts of the HPLC system. If the column re-equilibration after each run is not enough to...