This Information Applies To: Agilent 1100, 1200, 1260 LC Pumps.
The following procedure allows the cleaning of residue from the pump's check valves, often caused by polymerization of aged acetonitrile or salt-based solutions that may prevent the inlet and outlet valves from working correctly.
When inlet and/or outlet valves do not close correctly, the pump pressure will fluctuate. When monitoring the pressure trace, the user may see a sawtooth pattern (up and down) of various amplitudes.
If the sonication of the valves does not solve the issue, please contact Agilent Technologies for further support.
Steps to follow:
Figure 1. Outlet Valve
The inlet valve is positioned below the pump head as shown in Figure 2. It shows an Active Inlet Valve.
Figure 2. Active Inlet Valve
The inlet valve can be either passive or active.
To disconnect the Active Inlet Valve, follow these steps:
Figure 3. Active Inlet Valve Cartridge.
The Passive Inlet Valve can be disconnected similarly to the outlet one, and it will not need to be further disassembled before sonication.
Learn how to effectively troubleshoot your HPLC pump:
HPLC-0GEN-2201e - Chromatographic Troubleshooting for HPLC e-learning course available from Agilent education