Hi all :) For he experts here! I did my yearly maintenance (new gold seal, liner, column end cut to inlet and FID, new gas filters, ...). But since then, everytime there's a temperature change in the GC, the signal will show a sudden bump and then it…
I'm running a GC(7890A) / MS(5975C) with MassHunter Acquisition software. I automate much of my data analysis in Python by exporting the GC signal (FID) to .csv, and then exporting the MS signal using 'Export 3-D Data...' in MSD Productivity Chemstation…
Hello MikeL ,
Just wanted to post what we discussed during your call with the support center so if anyone else sees a similar problem they will have some things to try.
There can be issues with copying and pasting fairly large amounts of information…
Recently, we connected the column (CP7429) to TCD and FID using a Y splitter. However, there is no signal response on both TCD and FID after manually injection of the calibration gas (containing H2, CO, CH4, C2H4, C2H6 in CO2). The carrier gas is Ar.…
i had faced this troubleshooting before
it is probably a software problem.
first close masshunter and double click ( remove masshunter process)
open the soft again and try to create another method with same parameters it should work
also try to…
When performing the Eukariote Total RNA test on the same chip we had some samples with very degraded RNA and one sample with what seems like good quality RNA but the software failed to give a RIN value for that sample. Is there a way to still measure…
Dear Colleagues, apologizing for frequently wondering but I first faced this problem. GC produces a signal without turning on the FID Detector!? Please look at the attached photo. After turning on the detector and then conditioning the column while the…
For many FID methods, 10-20 Hz data rate is sufficient unless you're doing a fast method of some sort. You need enough data points to define the peak correctly and not get any spectral tilt. Typically 15-20 points across the peak is enough.
Hello AGILENT family,
i have a few questions on the analysis of Pb in blood samples.
am failing to get the right concentrations of the QC sample 8ppb instead it’s reading 0.08 ppb and at the same time it’s also showing overange (0.08o) even when my…
I am a user of an Agilent 7900 ICP-MS. It has been working fine since last month, but today (Oct 13, 2022) the software was not able to connect to the instrument. When I try to connect the MassHunter software to the ICPMS by going to settings > mainframe…
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Issue
The Agilent 2100 Bioanalyzer system run ends prematurely and the following error appears in the logbook: Run aborted on port x. Instrument error occurred on port x, Unusual...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Issue
The Agilent 2100 Bioanalyzer system electropherograms in the Agilent 2100 Expert software have a wavy, uneven baseline ( Figure 1 ).
Figure 1. Wavy, uneven baseline on Bioanalyzer...
This Information Applies To: Agilent AriaMx systems.
Issue
When IP addresses of an AriaMx instrument are reassigned, it will appear under the new and the previous IP address in the AriaMx Instrument Explorer. This situation can be prevented using...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
Sometimes, for support purposes, it is necessary to send the diagnostic test result file to Agilent support representatives.
This file contains detailed information about the...
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx Real-Time PCR software
Issue
How to change the discovery protocol port in the AriaMx software.
Steps to Follow
The AriaMx software uses the UDP Broadcast protocol for...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
The instrument diagnostic tests should be run whenever it is suspected that there could be a fault with the instrument hardware.
Background
The instrument diagnostics consist...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
Error 45 is displayed on the AriaMx instrument touch screen.
Background
Error 45 is shown when the temperature recorded by one or more thermistors is outside the expected...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
AriaMx showing a series of errors after delivery, guide for troubleshooting and resolving temperature-related error.
Background
When an AriaMx is delivered, it could take...
How to Access Manual Tune in GC/MS MassHunter and MSD Productivity ChemStation
Multiple Results
This Information Applies To: MassHunter acquisition software for GC/MS systems and MSD Productivity ChemStation for all Agilent GC/MS systems
Issue
Manual tuning lets you interactively set the MSD parameters, such as lens voltages and tuning masses...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
How to find the AriaMx software and firmware version that you have installed.
Steps to Follow
There are two different ways to find out AriaMx software version:
In the...