Sorry for the delayed response. I've found that either works fine. I typically try to keep injection volume low (0.2-1 uL for 1uM protein). I've mostly been using 50mM HEPES 150mM NaCl and get great deconvolution results, even with a 3 min short method…
I am getting message "draw command failed, EE34205" while running batch in G7129B infinity 2 autosampler. I have tried replacing transport assembly, but error is same. I have used another module G7129A Infinity autosampler with this LC, but same error…
Hello all! We are currently familiarising ourselves with the MassHunter data aquisition software (LC/MS Data Aquisition for 6200 series TOF/6500 series Q-TOF. Version B.08.00 Build 8.00.8058.3 SP1). We have been trying to find the most efficient way of…
How do you set up an auditory sound alert for when a method run completes? I was thinking it may have been a runtime event but I I can’t see my to find it .I set a alarm on my phone for the length of the experiment to remind myself to go check and set…
Lines 3-5 (blank, blank, 1/100ppb) make me think there is a contamination issue. Check the cps of Ni on those first 4 blanks. If you see a downward trend, that could be an indication of a Nickel contamination somewhere in the instrument that is washing…
For Research Use Only. Not for use in diagnostic procedures.
Here is a "best of" collection of links to manuals and tools for Agilent Automated Electrophoresis products (Bioanalyzer, TapeStation, Fragment Analyzer, ZAG DNA Analyzer, Femto Pulse, Oligo…
Hello,
My name is Ben Niemi from the New Hampshire Public Health Laboratories. We are currently running 6470 QQQ LC-MS/MS with a 1260 Infinity II LC Stack.
Recently we've encountered some condensation issues in our column compartment on the line that…
Recommendations:
Increase the Oven Temperature equilibration time from 0.5 minutes to 1 to 1.5 minutes. The GC oven walls need more time to cool down from 300° C back to 50° C to be ready for the next run. This affects run-to-run reproducibility.
…
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Issue
The Agilent 2100 Bioanalyzer system run ends prematurely and the following error appears in the logbook: Run aborted on port x. Instrument error occurred on port x, Unusual...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Issue
The Agilent 2100 Bioanalyzer system electropherograms in the Agilent 2100 Expert software have a wavy, uneven baseline ( Figure 1 ).
Figure 1. Wavy, uneven baseline on Bioanalyzer...
This Information Applies To: Agilent AriaMx systems.
Issue
When IP addresses of an AriaMx instrument are reassigned, it will appear under the new and the previous IP address in the AriaMx Instrument Explorer. This situation can be prevented using...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
Sometimes, for support purposes, it is necessary to send the diagnostic test result file to Agilent support representatives.
This file contains detailed information about the...
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx Real-Time PCR software
Issue
How to change the discovery protocol port in the AriaMx software.
Steps to Follow
The AriaMx software uses the UDP Broadcast protocol for...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
The instrument diagnostic tests should be run whenever it is suspected that there could be a fault with the instrument hardware.
Background
The instrument diagnostics consist...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
Error 45 is displayed on the AriaMx instrument touch screen.
Background
Error 45 is shown when the temperature recorded by one or more thermistors is outside the expected...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
AriaMx showing a series of errors after delivery, guide for troubleshooting and resolving temperature-related error.
Background
When an AriaMx is delivered, it could take...
How to Access Manual Tune in GC/MS MassHunter and MSD Productivity ChemStation
Multiple Results
This Information Applies To: MassHunter acquisition software for GC/MS systems and MSD Productivity ChemStation for all Agilent GC/MS systems
Issue
Manual tuning lets you interactively set the MSD parameters, such as lens voltages and tuning masses...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
How to find the AriaMx software and firmware version that you have installed.
Steps to Follow
There are two different ways to find out AriaMx software version:
In the...