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Cendy.DFor Research Use Only. Not for use in diagnostic procedures.
Here is a "best of" collection of links to manuals and tools for Agilent Automated Electrophoresis products (Bioanalyzer, TapeStation, Fragment Analyzer, ZAG DNA Analyzer, Femto Pulse, Oligo Pro II). For other instruments refer to Collection of Support Documents.
Automated Electrophoresis Resources
Agilent Automated Electrophoresis Portfolio
Table of Contents
- Webinar Notifications
- Product Updates
- 2100 Bioanalyzer
- 4200 TapeStation
- 4150 TapeStation
- 2200 TapeStation
- Fragment Analyzer
- ZAG DNA Analyzer
- Femto Pulse
- Oligo Pro II
Discontinuation of Legacy Fragment Analyzer Kits (in 2021)
General
2100 Expert Software User's Guide
2100 Bioanalyzer Maintenance and Troubleshooting Guide
2100 Bioanalyzer Site Preparation Checklist
2100 Bioanalyzer Software B.02.11 SR1 - Download link
2100 Bioanalyzer ReadMe file - B.02.11 SR1 Software Installation and PC System Requirements
2100 Bioanalyzer installation and PC connection instructions
2100 Bioanalyzer Compliance Solution
2100 Bioanalyzer and TapeStation Systems Consumables
Kit Guides
DNA 7500 and DNA 12000 kit guide
High Sensitivity DNA kit guide
High Sensitivity Protein 250 kit guide
Technical and Application Notes
DNA
2100 Bioanalyzer DNA Kits Overview
Comparison of Bioanalyzer and UV based Quantitation of QPCR Standards
2100 Bioanalyzer for Basmati Rice Authenticity Testing
Sample QC using Bioanalyzer and TapeStation for NGS Solutions
FFPE sample QC using Bioanalyzer
NGS library prep QC using 4150 TapeStation and 2100 Bioanalyzer
Comparison of DNA Assays Using the 4200 TapeStation System and 2100 Bioanalyzer System
RNA
2100 Bioanalyzer RNA Kits Overview
Quantitation Comparison of total RNA using Bioanalyzer, RiboGreen and UV
Quality Control of Guide RNA for CRISPR
DV200 Determination Tool and DV200 Technical Overview
Protein
2100 Bioanalyzer Protein Kits Overview
Protein Assays Portfolio with Data
Analysis of Glycosylated Proteins on the Bioanalyzer
Maintenance and Troubleshooting
2100 Bioanalyzer : How To Video - Priming Station Maintenance
2100 Bioanalyzer : How to Video - Prepare a Chip
2100 Bioanalyzer : How To Video - Exchanging Cartridges
2100 Bioanalyzer: How To Video - Electrode Cartridge Maintenance
General
4200 TapeStation Site-Preparation Checklist
4200 TapeStation Compliance Solutions
4200 Software 4.1.1 - Download here
4200 Software ReadMe file - Installation and PC Requirements
LIMS Integration of the 4200 TapeStation System
4200 TapeStation Consumables and Reagents
Kit Guides
DNA
HSD1000 ScreenTape assay guide
HSD5000 ScreenTape assay guide
Genomic DNA ScreenTape assay guide
Cell-free DNA ScreenTape assay guide
RNA
Technical and Application Notes
DNA
Genomic DNA ScreenTape Data Sheet
D5000 ScreenTape and High Sensitivity D5000 ScreenTape Assays Data Sheet
Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays on 4200 TapeStation System
Agilent D1000 ScreenTape and High Sensitivity D1000 ScreenTape Data Sheet
Performance of the D1000 and High Sensitivity D1000 ScreenTape Assays on 4200 TapeStation System
Sample QC during Agilent SureSelectQXT WGS library preparation
Sample QC using Bioanalyzer and TapeStation for NGS Solutions.
QC of Magnis SureSelect Workflow using Agilent Automated Electrophoresis Platforms
Performance Characteristics of Cell-free DNA ScreenTape Assay
Sample QC with Cell-free DNA ScreenTape Assay
Comparison of DNA Assays Using the 4200 TapeStation System and 2100 Bioanalyzer System
RNA
RNA Quality Control on Tapestation systems
Performance of the RNA ScreenTape Assays on the 4200 TapeStation System
DV200 Evaluation with RNA ScreenTape Assays
Maintenance and Troubleshooting
4200 TapeStation Troubleshooting (starting page 153)
4200 TapeStation Troubleshooting Software (starting page 155)
4200 TapeStation Troubleshooting DNA Applications (starting page 162)
4200 TapeStation Troubleshooting RNA Applications (starting page 183)
4200 TapeStation Troubleshooting Instrument Communication (starting page 196)
Running Hardware Diagnostics and Collecting Troubleshooting Information (starting page 205)
4200 TapeStation Good Measurement Practices (starting page 81)
Cleaning the Instrument (starting page 136)
Engage the Transportation Lock (starting page 139)
Assembling Nest covers steps (starting page 145) and also listed in Agilent Information Center (AIC) (Start Menu>Agilent TapeStation Software>AIC)
Check correct Insertion of lower nest cover (page 152) and animation in Agilent Information Center (AIC) (Start Menu>Agilent TapeStation Software>AIC)
General
4150 TapeStation Site-Preparation Checklist
4150 TapeStation Software 4.1.1 - Download here
4150 Software ReadMe file - Installation and PC Requirements
LIMS Integration of the 4150 TapeStation System
4150 TapeStation Consumables and Reagents
Kit Guides
DNA
HSD1000 ScreenTape assay guide
HSD5000 ScreenTape assay guide
Genomic DNA ScreenTape assay guide
Cell-free DNA ScreenTape assay guide
RNA
Technical and Application Notes
DNA
Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays on 4150 TapeStation System
Performance of the D1000 and High Sensitivity D1000 ScreenTape Assays on 4150 TapeStation System
Performance of the Genomic DNA ScreenTape Assay on 4150 TapeStation System
QC of Magnis Sureselect Workflow using Agilent Automated Electrophoresis Platforms
Sample QC using Bioanalyzer and TapeStation for NGS Solutions.
NGS library QC using 4150 TapeStation and 2100 Bioanalyzer at different steps
Performance Characteristics of Cell-free DNA ScreenTape Assay
Sample QC with Cell-free DNA ScreenTape Assay
RNA
RNA Quality Control on Tapestation systems
Performance of the RNA and High Sensitivity RNA ScreenTape Assays on 4150 TapeStation System
DV200 Evaluation with RNA ScreenTape Assays
Maintenance and Troubleshooting
4150 TapeStation Troubleshooting Software (starting page 169)
4150 TapeStation Troubleshooting DNA Applications (starting page 180)
4150 TapeStation Troubleshooting RNA Applications (starting page 202)
4150 TapeStation Troubleshooting Instrument Communication (starting page 215)
Running Hardware Diagnostics on 4150 TapeStation (starting page 115)
Collecting 4150 TapeStation Troubleshooting Information (starting page 224)
4150 TapeStation Good Measurement Practices (starting page 81)
4150 TapeStation Maintenance Details (starting page 125)
Cleaning the Instrument (starting page 148)
Engage the Transportation Lock (starting page 149)
Assembling and Inserting Nest Covers steps (starting page 158) and also listed in Agilent Information Center (AIC) (Start Menu>Agilent TapeStation Software>AIC)
4200 TapeStation manual>Check Correct Insertion of Lower Nest Cover (page 165) and animation in Agilent Information Center (AIC) (Start Menu>Agilent TapeStation Software>AIC)
General
2200 TapeStation Software A.02.02 SR1 - Download here
2200 Software ReadMe file - Installation and PC Requirements
Kit Guides
DNA
HSD1000 ScreenTape assay guide
HSD5000 ScreenTape assay guide
Genomic DNA ScreenTape assay guide
Cell-free DNA ScreenTape assay is NOT available for 2200 Tapestation
RNA
Technical and Application Notes
DNA
Performance of the D1000 and High Sensitivity D1000 ScreenTape Assays on 2200 TapeStation System
Analysis of genomic DNA on 2200 Tapestation
RNA
Performance of the RNA ScreenTape Assays on the 2200 TapeStation System
DV200 Evaluation with RNA ScreenTape Assays
Maintenance and Troubleshooting
2200 TapeStation Troubleshooting Manual
2200 TapeStation Troubleshooting Instrument Communication (starting page 23)
2200 TapeStation Troubleshooting DNA Applications (starting page 39)
2200 TapeStation Troubleshooting RNA Applications (starting page 67)
2200 TapeStation Troubleshooting Error Messages (starting page 93)
Collecting 2200 TapeStation Troubleshooting Information (starting page 34)
2200 TapeStation Good Measurement Practices
2200 TapeStation Essential Measurement Practices (starting page 5)
2200 TapeStation Maintenance Details (starting page 85)
General
Fragment Analyzer Controller Software 3.1.0.12 (and Drivers)
ProSize (4.0.0.3) Data Analysis Software
FFPE DV200 Calculation Method/Configuration File
CRISPR Plug-in Configuration File
Fragment Analyzer Consumables, Array Options
Nucleic Acid analysis using 5200, 5300, and 5400 Fragment Analyzer Systems
Array Formats for Parallel CE Instruments
Kit Guides
DNA Quantitative
HS Large Fragment 50 kb Kit- DNF-464-0500
Large Fragment kit - DNF-493 (to be discontinued on Sep 1, 2021)
Genomic DNA 50 kb Kit - DNF-467-0500
HS Genomic DNA 50 kb Kit - DNF-468-0500
NGS Fragment Kit - DNF-473-0500
NGS Fragment Kit - DNF-473-1000
Small Fragment Kit - DNF-476-0500
HS Small Fragment Kit - DNF-477(To be discontinued on Mar 1, 2021)
DNA Qualitative
dsDNA 905 Reagent Kit - DNF-905 dsDNA Kit (1-500 bp)
dsDNA 935 Reagent Kit - DNF-935 Reagent Kit (1 – 1500 bp)
dsDNA 910 Reagent Kit- DNF-910
dsDNA 915 Reagent Kit - DNF-915
dsDNA 920 Reagent Kit - DNF-920
dsDNA 930 Reagent Kit - DNF-930
CRISPR DIscovery kit - DNF- 910
Plasmid DNA Kit - DNF-940-K0500
RNA Quantitative
Technical and Application Notes
Best Practices with an Agilent Parallel CE System
Quantification using 5200 Fragment Analyzer System
Sizing using 5200 Fragment Analyzer System
DNA
Genomic DNA Quality assessment using 5200 Fragment Analyzer
QC of cell free DNA using 5200 Fragment Analyzer
RNA
Eukaryotic Total RNA QC on 5200 Fragment Analyzer using HS RNA Kit (DNF‑472) and RNA Kit (DNF-471)
QC of IVT RNA on 5200 Fragment Analyzer using HS RNA Kit (DNF-472) and RNA Kit (DNF‑471)
Assessment of long IVT mRNA on 5200 Fragment Analyzer using DNF-471 kit
Maintenance and Troubleshooting
Preventative Maintenance Schedule (page 145)
Capillary Array Alignment (starting page 52)
Capillary Array Cleaning (starting page 126)
Capillary Array Window Cleaning (page 153)
Collecting Troubleshooting information - Event, Error reports and Zipped data folder.
Troubleshooting High back pressure
General
ZAG Controller Software 2.1.04 (with Drivers)
ProSize (4.0.0.3) Data Analysis Software
ZAG Capillary Array Uninstall, Unpack and Install steps, Page 85
ZAG Analyzer Consumables (Analysis Kits and Arrays)
Long Term storage of Parallel CE instruments
Syringe Replacement for Parallel CE instruments
Kit Guides
ZAG 110 dsDNA Kit (35–5000 bp)
ZAG 130 dsDNA Kit (75 - 20000 bp)
Technical and Application Notes
DNA
DNA Fragment Sizing with the ZAG
Sizing Resolution on ZAG using 105 dsDNA Kit
Maintenance and Troubleshooting
ZAG Preventative Maintenance Schedule, Page 142
ZAG Capillary Array Uninstall, Install, Page 85
ZAG Capillary Array Cleaning, Page 144
Collecting Troubleshooting Information - Event and Error Report (starting page 46)
Troubleshooting High back pressure issue
Syringe replacement for parallel CE instruments
General
Femto Pulse Controller Software 2.0.0.3 (with Drivers)
ProSize (4.0.0.3) Data Analysis Software
Femto Pulse User Manual - Capillary Array Uninstall, Install (Chapter 8)
Kit Guides
DNA
Genomic DNA 165 kb Kit - FP-1002-0275
Ultra Sensitivity NGS Kit - FP-1101-0275
RNA
Ultra Sensitivity RNA Kit - FP-1201-0275
Technical and Application Notes
DNA
Quantify and Qualify DNA Fragments using FP-1002 gDNA 165 Kb Analysis Kit on the Femto Pulse system
Genomic DNA QC using Genomic DNA 165 kb Kit (FP-1002-0275) on the Femto Pulse System
GQN Assessment of Genomic DNA using Genomic DNA 165 kb Kit (FP-1002-0275) on the Femto Pulse System
Ultra-Sensitivity NGS Analysis Kit (FP-1101) on the Femto Pulse System
Cell free DNA QC using Ultra Sensitivity NGS Kit (FP-1101) on the Femto Pulse System
RNA
Ultra-Sensitivity RNA Analysis Kit (FP-1201) and the Femto Pulse System
Maintenance and Troubleshooting
Femto Pulse User Manual - Capillary Array Uninstall, Install (Chapter 8)
Syringe replacement for parallel CE instruments
Long Term storage of Parallel CE instruments
Collecting Troubleshooting information - Event, Error reports and Zipped data folder
General
Oligo Pro II Controller Software 2.1.0.2 (with Drivers)
Oligo Pro II Data Analysis Software 2.0.0.3
Oligo Pro II Instrument User Manual
Oligo Pro II Data Analysis Software User Manual
Oligo Pro II Reagent Structure
Oligonucleotide Purity Assessment on Oligo Pro II System
Maintenance and Troubleshooting
Oligo Pro II Capillary Array Uninstall and Install, Page 90
Long Term storage of Parallel CE instruments
Collecting Troubleshooting information - Event, Error reports and Zipped data folder