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Collection of Automated Electrophoresis Resources

Created by Shweta.S Shweta.S over 1 year ago | Last modified by Cendy.D Cendy.D 1 month ago

For Research Use Only. Not for use in diagnostic procedures.

Here is a "best of" collection of links to manuals and tools for Agilent Automated Electrophoresis products (Bioanalyzer, TapeStation, Fragment Analyzer, ZAG DNA Analyzer, Femto Pulse, Oligo Pro II). For other instruments refer to Collection of Support Documents.

 

Automated Electrophoresis User Manuals and Support Resources

Automated Electrophoresis Resources

Agilent Automated Electrophoresis Portfolio

Table of Contents

  • Webinar Notifications 
  • Product Updates 
  • 2100 Bioanalyzer
  • 4200 TapeStation
  • 4150 TapeStation
  • 2200 TapeStation
  • Fragment Analyzer
  • ZAG DNA Analyzer
  • Femto Pulse
  • Oligo Pro II

Product Updates 

Discontinuation of Legacy Fragment Analyzer Kits (in 2021)

2100 Bioanalyzer

General

2100 Expert Software User's Guide 

2100 Bioanalyzer Maintenance and Troubleshooting Guide

2100 Bioanalyzer Site Preparation Checklist 

2100 Bioanalyzer Software B.02.11 SR1 - Download link

2100 Bioanalyzer ReadMe file - B.02.11 SR1 Software Installation and PC System Requirements

2100 Bioanalyzer installation and PC connection instructions

2100 Bioanalyzer Compliance Solution

2100 Bioanalyzer Accessories

2100 Bioanalyzer and TapeStation Systems Consumables

Kit Guides

RNA 6000 Nano kit guide

RNA 6000 Pico kit guide

Small RNA kit guide

DNA 1000 kit guide  

DNA 7500 and DNA 12000 kit guide

High Sensitivity DNA kit guide  

Protein 80 kit guide

Protein 230 kit guide

High Sensitivity Protein 250 kit guide

Technical and Application Notes 

DNA

2100 Bioanalyzer DNA Kits Overview

Comparison of Bioanalyzer and UV based Quantitation of QPCR Standards

2100 Bioanalyzer for Basmati Rice Authenticity Testing

Sample QC using Bioanalyzer and TapeStation for NGS Solutions

FFPE sample QC using Bioanalyzer 

NGS library prep QC using 4150 TapeStation and 2100 Bioanalyzer

Comparison of DNA Assays Using the 4200 TapeStation System and 2100 Bioanalyzer System

RNA

2100 Bioanalyzer RNA Kits Overview

Small RNA Analysis

RNA Integrity Number (RIN)

Plant RNA RIN

Insect RNA RIN

Quantitation Comparison of total RNA using Bioanalyzer, RiboGreen and UV

Quality Control of Guide RNA for CRISPR

DV200 Determination Tool and DV200 Technical Overview

Protein

2100 Bioanalyzer Protein Kits Overview

Protein Assays Portfolio with Data

Analysis of Glycosylated Proteins on the Bioanalyzer

Maintenance and Troubleshooting

2100 Bioanalyzer Maintenance and Troubleshooting Guide -  RNA, DNA, Protein, Instrument Communication Related Issues

Running Hardware Diagnostics

2100 Bioanalyzer : How To Video - Priming Station Maintenance

2100 Bioanalyzer : How to Video - Prepare a Chip 

2100 Bioanalyzer : How To Video - Exchanging Cartridges

2100 Bioanalyzer: How To Video - Electrode Cartridge Maintenance

4200 TapeStation

General

4200 TapeStation User Manual

4200 TapeStation Site-Preparation Checklist

4200 TapeStation Compliance Solutions

4200 Software 4.1.1 - Download here

4200 Software ReadMe file - Installation and PC Requirements

LIMS Integration of the 4200 TapeStation System

4200 TapeStation Consumables and Reagents

4200 TapeStation Accessories

Kit Guides

DNA

D1000 ScreenTape assay guide

HSD1000 ScreenTape assay guide

D5000 ScreenTape assay guide

HSD5000 ScreenTape assay guide

Genomic DNA ScreenTape assay guide

Cell-free DNA ScreenTape assay guide

RNA

RNA ScreenTape assay guide 

HSRNA ScreenTape assay guide

Technical and Application Notes

DNA

DNA Integrity Number (DIN)

Genomic DNA ScreenTape Data Sheet

D5000 ScreenTape and High Sensitivity D5000 ScreenTape Assays Data Sheet

Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays on 4200 TapeStation System

Agilent D1000 ScreenTape and High Sensitivity D1000 ScreenTape Data Sheet

Performance of the D1000 and High Sensitivity D1000 ScreenTape Assays on 4200 TapeStation System

Sample QC during Agilent SureSelectQXT WGS library preparation

Sample QC using Bioanalyzer and TapeStation for NGS Solutions.

QC of Magnis SureSelect Workflow using Agilent Automated Electrophoresis Platforms

Cell-free DNA Data Sheet

Performance Characteristics of Cell-free DNA ScreenTape Assay 

Sample QC with Cell-free DNA ScreenTape Assay  

Comparison of DNA Assays Using the 4200 TapeStation System and 2100 Bioanalyzer System

RNA

RNA Quality Control on Tapestation systems

Performance of the RNA ScreenTape Assays on the 4200 TapeStation System

DV200 Evaluation with RNA ScreenTape Assays

Maintenance and Troubleshooting

4200 TapeStation Troubleshooting (starting page 153)

4200 TapeStation Troubleshooting Software (starting page 155)

4200 TapeStation Troubleshooting DNA Applications (starting page 162)

4200 TapeStation Troubleshooting RNA Applications (starting page 183)

4200 TapeStation Troubleshooting Instrument Communication (starting page 196)

Running Hardware Diagnostics and Collecting Troubleshooting Information (starting page 205)

4200 TapeStation Good Measurement Practices (starting page 81)   

Annual Preventive Maintenance (PM) Details (starting page 130) (PM to be executed by Agilent certified engineer)

Exchange the Piercing Needle (starting 131) (done during annual PM and can be done by users, if needed) 

Cleaning the Instrument (starting page 136) 

Engage the Transportation Lock (starting page 139)

Assembling Nest covers steps (starting page 145) and also listed in Agilent Information Center (AIC) (Start Menu>Agilent TapeStation Software>AIC)

Check correct Insertion of lower nest cover (page 152) and animation in Agilent Information Center (AIC) (Start Menu>Agilent TapeStation Software>AIC) 

4150 TapeStation

General

4150 TapeStation User Manual

4150 TapeStation Site-Preparation Checklist

4150 Compliance Solutions

4150 TapeStation Software 4.1.1 - Download here

4150 Software ReadMe file - Installation and PC Requirements

LIMS Integration of the 4150 TapeStation System

4150 TapeStation Consumables and Reagents

Kit Guides

DNA

D1000 ScreenTape assay guide

HSD1000 ScreenTape assay guide

D5000 ScreenTape assay guide

HSD5000 ScreenTape assay guide

Genomic DNA ScreenTape assay guide

Cell-free DNA ScreenTape assay guide

RNA

RNA ScreenTape assay guide 

HSRNA ScreenTape assay guide

Technical and Application Notes

DNA

DNA Integrity Number (DIN)

Performance of the D5000 and High Sensitivity D5000 ScreenTape Assays on 4150 TapeStation System

Performance of the D1000 and High Sensitivity D1000 ScreenTape Assays on 4150 TapeStation System

Performance of the Genomic DNA ScreenTape Assay on 4150 TapeStation System

QC of Magnis Sureselect Workflow using Agilent Automated Electrophoresis Platforms

Sample QC using Bioanalyzer and TapeStation for NGS Solutions.

NGS library QC using 4150 TapeStation and 2100 Bioanalyzer at different steps

Cell-free DNA Data Sheet

Performance Characteristics of Cell-free DNA ScreenTape Assay 

Sample QC with Cell-free DNA ScreenTape Assay  

RNA

RNA Quality Control on Tapestation systems

Performance of the RNA and High Sensitivity RNA ScreenTape Assays on 4150 TapeStation System

DV200 Evaluation with RNA ScreenTape Assays

Maintenance and Troubleshooting

4150 TapeStation Troubleshooting Software (starting page 169)

4150 TapeStation Troubleshooting DNA Applications (starting page 180)

4150 TapeStation Troubleshooting RNA Applications (starting page 202)

4150 TapeStation Troubleshooting Instrument Communication (starting page 215)

Running Hardware Diagnostics on 4150 TapeStation (starting page 115)

Collecting 4150 TapeStation Troubleshooting Information (starting page 224)

4150 TapeStation Good Measurement Practices (starting page 81)

4150 TapeStation Maintenance Details (starting page 125)

Exchange the Piercing Needle (starting page 129) (done during annual PM and can be done by users, if needed)

Exchange Electrode Cartridge (starting page 137) (done during annual PM and can be done by users, if doing self PM)

Cleaning the Instrument (starting page 148)

Engage the Transportation Lock (starting page 149)

Assembling and Inserting Nest Covers steps (starting page 158) and also listed in Agilent Information Center (AIC) (Start Menu>Agilent TapeStation Software>AIC)

4200 TapeStation manual>Check Correct Insertion of Lower Nest Cover (page 165) and animation in Agilent Information Center (AIC) (Start Menu>Agilent TapeStation Software>AIC) 

2200 TapeStation

General

2200 TapeStation User Manual

2200 TapeStation Software A.02.02 SR1 - Download here

2200 Software ReadMe file - Installation and PC Requirements

2200 TapeStation Accessories

Kit Guides

DNA

D1000 ScreenTape assay guide

HSD1000 ScreenTape assay guide

D5000 ScreenTape assay guide

HSD5000 ScreenTape assay guide

Genomic DNA ScreenTape assay guide

Cell-free DNA ScreenTape assay is NOT available for 2200 Tapestation

RNA

RNA ScreenTape assay guide

HSRNA ScreenTape assay guide

Technical and Application Notes

DNA

DNA Integrity Number (DIN)

Performance of the D1000 and High Sensitivity D1000 ScreenTape Assays on 2200 TapeStation System

Analysis of genomic DNA on 2200 Tapestation

RNA

Performance of the RNA ScreenTape Assays on the 2200 TapeStation System

DV200 Evaluation with RNA ScreenTape Assays

Maintenance and Troubleshooting

2200 TapeStation Troubleshooting Manual

2200 TapeStation Troubleshooting Instrument Communication (starting page 23)

2200 TapeStation Troubleshooting DNA Applications (starting page 39)

2200 TapeStation Troubleshooting RNA Applications (starting page 67)

2200 TapeStation Troubleshooting Error Messages (starting page 93)

Collecting 2200 TapeStation Troubleshooting Information (starting page 34)

2200 TapeStation Good Measurement Practices

2200 TapeStation Essential Measurement Practices (starting page 5)

2200 TapeStation Maintenance Details (starting page 85)

Exchange the Piercing Needle (starting page 87; done during annual PM and can be done by users, if needed)

Fragment Analyzer

General

Fragment Analyzer User Manual

Fragment Analyzer Controller Software 3.1.0.12 (and Drivers)

ProSize (4.0.0.3) Data Analysis Software

FFPE DV200 Calculation Method/Configuration File 

CRISPR Plug-in Configuration File

Fragment Analyzer Consumables, Array Options

Array Installation steps

Nucleic Acid analysis using 5200, 5300, and 5400 Fragment Analyzer Systems

Array Formats for Parallel CE Instruments

Steps for Long term storage of Fragment Analyzer, ZAG DNA analyzer, Femto Pulse and Oligo Pro II instruments

Kit Guides

DNA Quantitative

HS Large Fragment 50 kb Kit- DNF-464-0500

Large Fragment Kit - DNF-492

Large Fragment kit - DNF-493 (to be discontinued on Sep 1, 2021)

Genomic DNA 50 kb Kit - DNF-467-0500

HS Genomic DNA 50 kb Kit - DNF-468-0500

HS Genomic DNA Kit - DNF-488

NGS Fragment Kit - DNF-473-0500

NGS Fragment Kit - DNF-473-1000

HS NGS Fragment Kit - DNF-474

Small Fragment Kit - DNF-476-0500

HS Small Fragment Kit  - DNF-477(To be discontinued on Mar 1, 2021)

DNA Qualitative

dsDNA 905 Reagent Kit - DNF-905 dsDNA Kit (1-500 bp)

dsDNA 935 Reagent Kit - DNF-935 Reagent Kit (1 – 1500 bp)

dsDNA 910 Reagent Kit- DNF-910

dsDNA 915 Reagent Kit - DNF-915

dsDNA 920 Reagent Kit  - DNF-920

dsDNA 930 Reagent Kit - DNF-930

CRISPR DIscovery kit - DNF- 910

Plasmid DNA Kit - DNF-940-K0500

RNA Quantitative

Small RNA Kit - DNF-470-0275

RNA Kit - DNF-471

HS RNA Kit - DNF-472

Technical and Application Notes

Best Practices with an Agilent Parallel CE System

Quantification using 5200 Fragment Analyzer System

Sizing using 5200 Fragment Analyzer System

DNA

Genomic DNA Quality assessment using 5200 Fragment Analyzer 

QC of cell free DNA using 5200 Fragment Analyzer

Resolution (up to 3 bp) using dsDNA 905 kit (DNF-905) and HS small Fragment Kit (DNF-477) on 5200/5300 Fragment Analyzer

RNA

Eukaryotic Total RNA QC on 5200 Fragment Analyzer using HS RNA Kit (DNF‑472) and RNA Kit (DNF-471)

Single-Guide RNA (CRISPR) QC on 5200/5300/5400 Fragment Analyzer using HS RNA Kit (DNF- 472) and Small RNA Kit (DNF-470)

Comparison of HS Small Fragment Kit (DNF‑477) and HS NGS Fragment Kit (DNF‑474) on the Agilent 5200 Fragment Analyzer

QC of IVT RNA on 5200 Fragment Analyzer using HS RNA Kit (DNF-472) and RNA Kit (DNF‑471) 

Plant RNA Degradation Detection on 5200 Fragment Analyzer using HS RNA Kit (DNF-472) and RNA Kit (DNF-471)

Assessment of long IVT mRNA on 5200 Fragment Analyzer using DNF-471 kit 

Maintenance and Troubleshooting

Preventative Maintenance Schedule (page 145)

Capillary Array Alignment (starting page 52)

Capillary Array Cleaning (starting page 126)

Capillary Array Window Cleaning (page 153)

Syringe Replacement

Collecting Troubleshooting information - Event, Error reports and Zipped data folder. 

Troubleshooting High back pressure

ZAG DNA Analyzer

General

ZAG DNA Analyzer Manual

ZAG Controller Software 2.1.04 (with Drivers)

ProSize (4.0.0.3) Data Analysis Software

ZAG Capillary Array Uninstall, Unpack and Install steps, Page 85

ZAG Analyzer Consumables (Analysis Kits and Arrays)

Long Term storage of Parallel CE instruments

Syringe Replacement for Parallel CE instruments

Kit Guides 

ZAG 105 dsDNA Kit (1-500 bp)

ZAG 110 dsDNA Kit (35–5000 bp)

ZAG 130 dsDNA Kit (75 - 20000 bp)

ZAG 135 dsDNA Kit (1–1500 bp)

Technical and Application Notes

DNA

DNA Fragment Sizing with the ZAG

Genotyping using ZAG

Sizing Resolution on ZAG using 105 dsDNA Kit

Maintenance and Troubleshooting

ZAG Preventative Maintenance Schedule, Page 142

ZAG Capillary Array Uninstall, Install, Page 85

ZAG Capillary Array Cleaning, Page 144

Collecting Troubleshooting Information - Event and Error Report (starting page 46)
Troubleshooting High back pressure issue

Syringe replacement for parallel CE instruments

Femto Pulse

General

Femto Pulse User Manual  

Femto Pulse System Brochure

Femto Pulse Controller Software 2.0.0.3 (with Drivers)

ProSize (4.0.0.3) Data Analysis Software

Femto Pulse kits

Femto Pulse User Manual - Capillary Array Uninstall, Install (Chapter 8)

Steps for Long term storage of Fragment Analyzer, ZAG DNA analyzer, Femto Pulse and Oligo Pro II instruments

Kit Guides 

DNA

Genomic DNA 165 kb Kit - FP-1002-0275

Ultra Sensitivity NGS Kit - FP-1101-0275

55 kb BAC Kit - FP-1003-0275

RNA

Ultra Sensitivity RNA Kit - FP-1201-0275

Technical and Application Notes

DNA

Quantify and Qualify DNA Fragments using FP-1002 gDNA 165 Kb Analysis Kit on the Femto Pulse system

Genomic DNA QC using Genomic DNA 165 kb Kit (FP-1002-0275) on the Femto Pulse System

GQN Assessment of Genomic DNA using Genomic DNA 165 kb Kit (FP-1002-0275) on the Femto Pulse System

Ultra-Sensitivity NGS Analysis Kit (FP-1101) on the Femto Pulse System

Cell free DNA QC using Ultra Sensitivity NGS Kit (FP-1101) on the Femto Pulse System

RNA

Ultra-Sensitivity RNA Analysis Kit (FP-1201) and the Femto Pulse System

Maintenance and Troubleshooting

Femto Pulse User Manual - Capillary Array Uninstall, Install (Chapter 8)

Syringe replacement for parallel CE instruments

Long Term storage of Parallel CE instruments

Collecting Troubleshooting information - Event, Error reports and Zipped data folder

Oligo II

General

Oligo Pro II Controller Software 2.1.0.2 (with Drivers)

Oligo Pro II Data Analysis Software 2.0.0.3

Oligo Pro II Instrument User Manual

Oligo Pro II Data Analysis Software User Manual

Oligo Pro II Reagent Structure 

Oligonucleotide Purity Assessment on Oligo Pro II System

Maintenance and Troubleshooting

Oligo Pro II Capillary Array Uninstall and Install, Page 90

Long Term storage of Parallel CE instruments

Collecting Troubleshooting information - Event, Error reports and Zipped data folder

  • 2200 TapeStation
  • ZAG DNA Analyzer
  • 5300 Fragment Analyzer
  • Troubleshooting
  • zipped
  • 2100
  • array uninstall
  • Femto Pulse
  • 5400 Fragment Analyzer
  • error report
  • 2200
  • event report
  • 2100 bioanalyzer communication
  • 5200
  • array install
  • 4200 TapeStation
  • zipped data file
  • 4150
  • 5400
  • 2100 Bioanalyzer
  • 4150 TapeStation
  • log files
  • manual
  • 5200 Fragment Analyzer
  • Oligo Pro II
  • 5300
  • array
  • 4200
  • support package
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