I am using OpenLab Chemstation C.01.10 on an 8890 with dual FIDs. I was trained on HP's version of Chemstation that reported both detector quant amounts in one row for precision purposes. It made it 10x easier to review data and confirm sample concentrations…
Thank you for the help so far. I was able to solve the problem on my results table by using the "left outer join" function.
However, the report will not print the individual peak chromatograms for the target ion or qualifiers if there was no peak detected…
We suddenly lost power during sample injection, when the power back, the autosampler got needle lock fail error message and fail to go back to needle seat, anybody could help? we did a cold start but not work.
Hi vitiin96,
this is very generic and any book on GC troubleshooting or the videos on the Agilent website could help. For the 7890 there is a troubleshooting manual available.
Good practice in GC is when you get a (new) column, you have an own suitability…
Hi Juliana
Yes, most likely the capacitors and/or the RF Tube. However, you can try to reduce the run power to 1.1Kw in the default ignition table. You may then be able to run for a few weeks with this reduced power level. You will need to reduce the…
Using the Agilent 7890A. Column installed with no leaks detected. Both the front and back detector are set to 300°C, gas Flow Rates - Hydrogen: 45mL/min, Air 450mL/min and He 1.0mL/min. The split flow is 80mL/min. Following initial start up the front…
I have started using GC-FID (Agilent 8890) since March for the first time. We are trying to measure the methane. I was trying to make a standard surve, so I injected 1000 ppm methane standard at first. Just to let you guys know no samples has been run…
Hello Everyone,
I am using 7820A GC coupled with 5977B Mass Spec. After use I close the He tank and later on I get 2 messages - 'Front inlet flow shutdown' and 'Front inlet pressure shutdown'. I restarted the software but it fails to load any GC method…
Varian 710 oes, nebulization is ok, fog in the chamber is omogeneous, tubing, nebulizer, torch, cone cleaned and alligned.
Work fine till 2 weeks ago, after i've experienced this: fail to execute wavelenght calibration end torch allign, No response…
Hello, we are setting up a seahorse assay for a immunology master class. We want to measure the glycolytic rate in human PBMCs or purified human CD4 T cells activated vs non activated. We fail to see any difference. Has anyone done this kind of assay…
This Information Applies To: Agilent 6820, 6890, 7820A GC systems with a Split/Splitless (SSL) inlet
Issue
Non-volatile sample matrix components will accumulate in the inlet liner and impact on analytical performance. The inlet liner should be changed...
This Information Applies To: Agilent headspace samplers (HS)
Issue
GC cycle time needs to be calculated correctly. Too short a GC cycle time will reduce sample quality and possibly stop the analysis prematurely. Too long GC cycle time can cause...
This Information Applies To: Agilent 7820A GC
Issue
7820A GC is designed with simplified keypad that comes with five buttons. They are Stop, Prep Run , Start, Up and Down arrow buttons. Changing the IP address requires a different method compared...
This Information Applies To: Agilent Single Needle Autosamplers
Background
Sometimes the user may observe liquid flowing from Port four on the autosampler injection valve. During an injection sequence, it is normal to have liquid from port four...
This Information Applies To: Agilent 4200, 4150, 2200 TapeStation systems
Issue
Can I run an assay using the ladder or sample buffer from a different assay if I have run out of the appropriate assay ladder or sample buffer? Is it possible to run...
This Information Applies To: Agilent ICP-MS 7700, 7800, 7850, 7900, 8800, and 8900 systems
Issue
Peristaltic pump tubing that has lost its elasticity will not deliver the sample to the nebulizer correctly. When the pump tubing appears limp or flattened...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
AriaMx touch screen displays error code 90 after the Instrument Health Check (IHC), which happens when the AriaMx is switched on, or after running the onboard diagnostic tests...
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx optical modules
Issue
AriaMx users can change which AriaMx optical modules are installed to upgrade the instrument capabilities or to troubleshoot.
Items Required
Any...
This Information Applies To: AriaMx real-time PCR system
Issue
Error 43 is displayed on the AriaMx touch screen. Error 43 is a voltage error and it normally indicates that the cartridge LED voltage or motor voltage being detected is abnormal. Sometimes...
This Information Applies To: Agilent 2100 Bioanalyzer system
Issue
Can I run qPCR samples containing Sybr® Green I on the Bioanalyzer?
Background
Sybr® Green I is an Intercalating Dye commonly used to detect amplification products in real...