Hello
I need to do an LC/MSMS method for quantification on a compound that doesn't ionize into [M+H]+ (barely observed) but only into [M-H2O+H]+
Is there a problem with doing MS/MS on a [M-H2O+H]+ parent ion ?
(PS: negative ionization is not an…
Hi again Raina,
as I already told you this cannot be done with just one column, especially with the CO2 concentration up to 20% and water included and the necessity to analyze H2. This is an application we normally build an analyzer for. You need at…
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Operating like this puts sub-optimal flow into the GCMS ion source of more than 2 ml/min. Please make sure that you have a system with a turbomolecular high vacuum pump and not a diffusion pump as 2 ml/min exceeds the maximum allowable flow into a diffusion…
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Hi Margot,
It may well be that the column performance has deteriorated so much within the 7 months that the EasiVial standards are measured with poorer PDI values. The column performance can be tested with regular plate count determinations. It is a…
Hi sojeong,
every test report, delivered with aour columns, contains multiple specifications, but they are all measured with specialized systems and software. That means you might not be able to reproduce them identically. BTW for plate count you also…
Hi, I cannot configure the column compartment (CC) as it came integrated with the vial sampler on LC1290. We have the G7129B model for the integrated vial sampler and CC. I tried adding the column compartment details from the instrument configuration…
When the tuning is done at 3200 (+) everything is fine. When I change it to tune at 1700 (+) the masses are reasonably accurate. After it gets to the third mass (620) it appears that the entire spectrum shifts by about 1.5 AMU and the tuning fails. There…
Hi Siddhartha,
Thank you for contacting Cell Analysis Technical Support. Can you please go ahead and send us your System Files so that we can see what might be going on? These can be accessed in the Help Section of the Wave software. You can select…
This Information Applies To: Agilent Atomic Absorption Spectroscopy Systems (240/280 Series Flame AA and 240/280Z/Zeeman/GTA 120 Graphite Furnace)
Issue:
This article includes recommended maintenance procedures to be performed daily, weekly or annually...
This Information Applies To: Agilent SpectrAA Software, Agilent 240/280 Flame Atomic Absorption Systems, 240/280 Zeeman Graphite Furnace Systems
Issue:
How to Reset Lamp Usage Counters or Add a New Lamp in SpectrAA
Caution : All physical...
This Information Applies To: Agilent GTA 120, Agilent 240Z AA Spectrometer, Agilent 280Z AA Spectrometer, Agilent 240FS/GTA120/PSD120/UltrAA System
Issue:
It is important to align the sample probe of the Programmable Sampler Dispenser (PSD) 120...
This Information Applies To: Agilent Flame AA Systems: e.g., 55B, 240FS, 280FS
Issue:
The Flame AA burner and burner components require regular cleaning to maintain system performance. Components include the burner, nebulizer, and spray chamber...
This Information Applies To: Agilent Flame Atomic Absorption (AA) Spectrometers (50/55/240/280 AA)
Issue:
How to clean a blocked nebulizer of a Flame AA, which may be causing low sensitivity or a sample uptake rate below 5 ml/min.
Steps to follow...
This Information Applies To: Agilent Graphite Furnace Systems (PSD 120/GTA 120/240Z AA/280Z AA)
Issue
Maintenance procedure for replacing the syringe and plunger of the PSD 120 sample dispenser for the Agilent GTA and Zeeman Graphite Furnace Systems...
This Information Applies To: Agilent AA (AA240, AA280) with Graphite Furnace Accessory (GTA)
Issue:
The Programmable Sample Dispenser (PSD) requires the sampling probe to be accurately aligned before the PSD can be used.
The PSD enables the...
This Information Applies To: All Agilent AA systems: 55B, 240FS, 280FS, 240Z, 280Z
Issue:
Optimizing the position of the lamp maximizes amount of light from the lamp passing through the optics to the detector, improving the signal-to-noise and the...
This Information Applies To: Agilent VGA 77, AA, VGA 77P, ICP
Issue:
The Agilent Vapor Generation Accessories VGA 77 and VGA 77P use high concentrations acids. The system needs to be thoroughly cleaned after each use.
The Vapor Generation Accessory...
This Information Applies To: All Agilent Atomic Absorption Spectrometry Systems (AA50, AA55, AA240, AA280)
Issue: This article describes when and how to clean an AA burner.
Background
For optimum and safe performance, burners (see Figure 1 ...