Hi howard_sanford, my apologies for a slow response, but thank you very much for the help and advice. I was able to get the filter for Cal and CC to work, and I appreciate the discussion of my larger problem. I think I'm probably several (many) steps…
Hello, thank you for your help. I had tried loading different processing methods but I didn't realize the results were not linked to any method. It turns out the result files were read-only and could not be linked to any method. I moved the result files…
The peaks look similar, essentially the same sensitivity. I'd look at the background on your older runs and the background now. It could be that most of the previous background was one, two, a few, ions and they're gone now. Was that small but still noticeable…
That is one failure mode of Platinum Resistance Thermometers. It can happen one degree to the next higher degree. The heater/sensor must be replaced, part number G1099-60107.
See also: (+) AUX 2 heater fault - Forum - GC/MS - Agilent Community
Hello,
My concern with chromatographic blank subtraction has to do more with the chromatography shifting in RT. When that happens even if the blank peak is smaller than the peak in the standard or sample it can shift enough to cause artifacts on your…
Yup, this seems like it'd be a super useful feature, except you'd have to make sure your CPS are adjusted for every batch after you calibrate. It would be nice to be able to just use the calibration curve for your batch
Hi, I don't think it is a fault of the purity of the solvent. This is normal when using acetonitrile. If you are using an iso pump to dose your reference masses, increase the flow rate, as the gradient rises. Another option is to change the mobile phase…
This Information Applies To: 5200/5300/5400 Fragment Analyzer, Femto Pulse, ZAG DNA Analyzer, ProSize Data Analysis Software
Issue
How to export a ladder as a size calibration file and import it into a different run in ProSize.
Background
...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue
It is possible to set up the 2100 Expert software so that the files are automatically exported each time a chip run is complete.
Steps to Follow
To turn...
This Information Applies To: 2100 Bioanalyzer system, 2100 Expert software
Issue:
How to identify and troubleshoot late migration of the ladder, marker, and sample peaks in data generated on the Agilent 2100 Bioanalyzer system.
Background
...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue
If communication between the 2100 Bioanalyzer and the PC cannot be established, or if an intermittent communication loss occurs, follow the instructions in...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue
Instructions on how to import from or export data into LIMS software.
Steps to Follow
Data import
When importing sample names and sample comments from...
This Information Applies To: 2100 Bioanalyzer system, 2100 Expert software version B.02.08 or later
Issue
When printing a report to PDF or HTML with the 2100 Expert software, the error "Failed to Generate Report" appears.
Background
This error...
This Information Applies To: 2100 Bioanalyzer system, 2100 Expert software
Issue
Resetting upper or lower markers from their automatically assigned settings in the Agilent 2100 Expert software.
Background
The Agilent 2100 Expert software...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert Software
Issue
Samples with a broad signal such as NGS smears should be analyzed by setting up a region. Analyzing a region is also termed smear analysis.
Steps to Follow...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software, RIN NA, Error 4501, RNA 6000 Nano kit (p/n 5067–1511 ), RNA 6000 Pico kit (p/n 5067–1513 )
Issue:
The RIN is not displayed in the software or shows as NA. If the RIN is NA, the...