• Created : 
  • Modified : 
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Automated Electrophoresis Software
  • Component :  Bioanalyzer Software
  • Product Name :  2100 Expert Security Pack 2100 Expert Software
  • Task :  Data Analysis Operation
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Reassigning the Upper or Lower Marker in the 2100 Bioanalyzer Expert Software

Answer

This Information Applies To: 2100 Bioanalyzer system, 2100 Expert software

Issue

Resetting upper or lower markers from their automatically assigned settings in the Agilent 2100 Expert software.

 

Background

The Agilent 2100 Expert software will assign the markers automatically. The software is able to assign the markers as they each have a known size (bp/nt/kDa) and therefore should migrate within a predetermined time frame. If a marker has not migrated within the expected time frame, or if there are other peaks close to the marker, a different peak may be incorrectly assigned as the marker. Incorrect marker peak assignment may result in inaccurate sizing and/or quantitation.

Steps to Follow

To check which peaks should be assigned as a marker:

  1. Open the file of interest in the 2100 Expert software.
  2. Select the Data context and turn off the analysis by clicking the button.
  3. To identify which peak should be set as the marker, view the electropherograms and the gel-like image.
  4. After you have identified the markers, turn the analysis back on by clicking the   button.

If you find that a marker peak has been set incorrectly:

  1. In the electropherogram view, select the sample of interest from the tree view and select the Peak Table.
  2. Hover the curser above the peak that you wish to assign as the upper or lower marker and right click.
  3. From the pop-up menu, select Manually Set Lower Marker or Manually Set Upper Marker as appropriate (Figure 1).
    Note: For more information regarding data analysis, please see the 2100 Expert software user's guide.

             

Figure 1. Assigning a peak as a marker in the 2100 Expert software

 

 
Learn how to effectively operate, maintain, and troubleshoot your Agilent 2100 Bioanalyzer system:
Agilent 2100 Bioanalyzer System Expert Software User's Guide on Agilent.com
Agilent 2100 Bioanalyzer System Maintenance and Troubleshooting Guide on Agilent.com 

For Research Use Only. Not for use in diagnostic procedures.

PR7001-0631

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