How to Set Up a Region in the 2100 Expert Bioanalyzer Software

Answer

This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert Software

Issue

Samples with a broad signal such as NGS smears should be analyzed by setting up a region. Analyzing a region is also termed smear analysis.

Steps to Follow

DNA, Small RNA, DV200 RNA, and Plant RNA assays

For these data files, smear analysis is automatically enabled. For these assays, to add a region to a single sample, you can add a region as described in the following instructions and shown in Figure 1.

1. Open the data file of interest and navigate to the sample that you wish to add a region for using the tree view in the 2100 Expert software.
2. Select the Region Table subtab in the Electropherogram tab.
3. Right click the electropherogram and select Add Region. 
4. Repeat the previous step until the number of required regions is inserted.
5. Adjust the regions by directly moving the region boundary lines on the electropherogram. Alternatively, right click and click Modify Regions. In the Smear Regions (Local Setpoints) menu, edit the From [bp], and To [bp], edit the region Name, and Color.

Figure 1. Setting up and editing a region for a single sample

To add a region to all samples within a data file follow the steps below, which are also shown in Figure 2.

1. Open the data file.  
2. If the Setpoint explorer is not visible on the right side of the software, click the View tab and click Setpoints.
3. In the Setpoint explorer, click the Global tab (If the Local tab is selected the region will only added for the selected sample). 
4. Select Advanced from the drop-down menu.
5. In the Smear Analysis section, double click Regions.
6. In the Smear Regions (Local Setpoints) menu, edit the From [bp], and To [bp], edit the region Name, and Color.

Figure 2.Setting up and editing a region for all samples in a file. 

Protein, mRNA, Eukaryotic and Prokaryotic Total RNA assays

For these data files, smear analysis must first be enabled in the setpoint viewer as described in the following instructions and shown in Figure 3.

1. Open the data file.  
2. If the Setpoint explorer is not visible on the right side of the software, click the View tab and click Setpoints.
3. In the Setpoint explorer, click the Global tab. 
4. Select Advanced from the drop-down menu. 
5. In the Smear Analysis section, select Perform Smear Analysis.
6. You can now follow the instructions in the DNA and Protein assays section.

Figure 3. Enabling smear analysis in the Setpoint Explorer

For more information on data analysis, see the 2100 Expert Software User's Guide.

Learn how to effectively operate, maintain, and troubleshoot your Agilent 2100 Bioanalyzer system: Agilent 2100 Bioanalyzer System Expert Software User's Guide on Agilent.com Agilent 2100 Bioanalyzer System Maintenance and Troubleshooting Guide on Agilent.com

For Research Use Only. Not for use in diagnostic procedures.

PR7001-1189