Hello Agilent Users,
Im missing a feature in Openlab since day 1. How do I override and start a run if the GC is not ready ?
For example: the FID temperature is set at 250°C in the method and the actual temperature is at 220°C (it is still heating…
The GC counter rolls over at 999,999...but 100,542 is still a lot!
Great drawing, thanks. That setup makes it difficult to figure out pressures/flows. The path of least resistance is all of the 530 pieces unless the methanizer and the connection from…
The idea behind the multiple modes is to keep the flows going through your FID constant. Changing flows will change the flame characteristics and cause the baseline to shift. Some suggestions:
If your column is set to constant flow mode, use "Constant…
We have a brand new Agilent 6495 LC/TQ system with a MS 120 rough pump. We vented and shut off the instrument for building maintenance over the weekend. On startup today, the rough pump receives power (the lights turn on) but eventually fails to engage…
Greetings Agilent Community,
Some MassHunter 10 difficulty. I am using the same Quant method that I have used multiple times in the past to process data. This time, however, I am experiencing an error while building my calibration curve. In the Methods…
Is there a table / document somewhere on what Agilent GC FID flows I should be using for a particular column ? Right now I have a 7890B with a 30m 0.32 column film thickness 0.25. We use it for petroleum / Diesel Range Organic analysis with a ALS injector…
I recently installed MassHunter Workstation B.03.01 SP2 on my PC to look over some old data that was obtained on a 6540 LC Q-TOF MS system. The instrument hasn't been used for a while now, and my lab no longer has the original computer. We just had this…
Andy G. had the excellent suggestion to decrease draw speed and wait time, since we are working with methanol extract. So we halved the draw speed from 100ul/min to 50ul/min and increased the wait time from 2 to 3 seconds. For now, this seems to have…
Hi,
We currently have as GC/MS/FID using Masshunter and need to print the FID traces as a pdf scaling the y-axis to zoom in on the minor peaks showing %Area values. Is this possible within any masshunter software? I've managed to get a report showing…
I'm not experienced in FID so I'm not sure what normal bleed looks like. So yes I would trim the column and check for leaks first then a new column. Can you look back at older runs when this column was installed to see what the bleed looks like to compare…
This Information Applies To: 5200/5300/5400 Fragment Analyzer, Femto Pulse, Oligo Pro II, and ZAG
Issue:
Only plasticware from the approved consumables list should be used in the Agilent Parallel Capillary Electrophoresis systems. The use of plasticware...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Question:
Can more than one Agilent 2100 Bioanalyzer instrument be connected to one PC?
Steps to follow:
It is possible to connect a maximum of two Bioanalyzers to one laptop...
This Information Applies To: 2100 Bioanalyzer, Bioanalyzer Chip Priming Station: part number 5065–4401
Maintenance procedures and how frequently they are required :
Procedure
Time interval
Or if this issue occurs
Where to find a detailed...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Issue:
Running the hardware diagnostics on the Agilent 2100 Bioanalyzer system can help to investigate whether the instrument performs as expected.
Steps to follow:
Required...
This Information Applies To: 2100 Bioanalyzer, RNA 6000 Nano kit, RNA 6000 Pico kit, Small RNA kit
Issue:
Is it possible to size RNA on the Agilent 2100 Bioanalyzer system?
Resolution:
Agilent offers total and small RNA assays on the Bioanalyzer...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue:
The Agilent 2100 Bioanalyzer system does not detect a prepared microfluidic chip after it is inserted into the instrument. The interface shows the instrument...
This Information Applies To: 2100 Bioanalyzer, Bioanalyzer chips
Issue:
Can 2100 Bioanalyzer assay chips and reagents be interchanged?
Resolution:
Some assay chips can be used with multiple assays, however some cannot because the chip layout...
This Information Applies To: Agilent MassHunter GC/MS Acquisition software
Issue
The MassHunter software performs several checks during the startup sequence. If it detects a problem, an alert message box will be displayed and the message bar on...
This Information Applies To: Agilent MassHunter Quantitative Data Analysis Software
Issue:
The "NO DATA POINTS" error message appears in the Compound Information window in the lower-left corner in Quantitative Analysis. Sometimes, this problem may...
This Information Applies To: Agilent GC/MSD MassHunter with MSD ChemStation Data Analysis
Issue
When you are using the Data Analysis Report, you may need to specify a certain number of decimal places for your results different to the default decimal...