Hi, could you please check if you have "Method Development" option activated in HS method settings? If you do, please set it to "None", save the method and try if it works. Thanks.
Hello sao.uch ,
There is not a way to do something like a spectral library match with chromatograms. If there are specific peaks you are interested in you could set up a simple quant method and define one run as your standard with a concentration of…
Here are some other helpful resources offered by Agilent:
Information on the 'Agilent Value Promise'
https://www.agilent.com/cs/library/brochures/brochure-agilent-value-promise-5991-1491en-agilent.pdf
Instrument lifetime information as it relates…
The GC is telling us that the front inlet pressure is 'Not Ready' is there a chance there is a leak in the inlet somewhere after the maintenance was performed?
Can the inlet hold pressure? If it can't hold pressure or does have a leak it will stop the…
Hello cpostigo ,
This error will occur if the batch has not been analyzed and there is no calibration curve available for your targets. The entries do appear to be accepted, and the calculation will be performed once the batch has been fully analyzed…
Hi, DP stands for Drug Product (I need to determine EtOH in a drug product not in a drug substance DS).
I'm loading 3 mL of sample in vial size 20 mL. The concentration of EtOH 4 mg/mL.
Yes of course, three replicates of three separate vials (same…
The Digest of upcoming webinars has been updated as of May 14.
The new Agilent Community Group, GPC/SEC Solutions Group hosted a webinar series in partnership with Dow in April: A Journey through SEC, Light Scattering, and 2D-LC for Macromolecules …
How can I create a method?
To create a method, settings can be selected on the setup page, e.g., wavelength range, averaging time, spectral bandwidth, etc. Some settings are application specific, e.g., kinetic, thermal. Baseline or zero correction can…
This Information Applies To: 5200/5300/5400 Fragment Analyzer, Femto Pulse, Oligo Pro II, and ZAG
Issue:
Only plasticware from the approved consumables list should be used in the Agilent Parallel Capillary Electrophoresis systems. The use of plasticware...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Question:
Can more than one Agilent 2100 Bioanalyzer instrument be connected to one PC?
Steps to follow:
It is possible to connect a maximum of two Bioanalyzers to one laptop...
This Information Applies To: 2100 Bioanalyzer, Bioanalyzer Chip Priming Station: part number 5065–4401
Maintenance procedures and how frequently they are required :
Procedure
Time interval
Or if this issue occurs
Where to find a detailed...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Issue:
Running the hardware diagnostics on the Agilent 2100 Bioanalyzer system can help to investigate whether the instrument performs as expected.
Steps to follow:
Required...
This Information Applies To: 2100 Bioanalyzer, RNA 6000 Nano kit, RNA 6000 Pico kit, Small RNA kit
Issue:
Is it possible to size RNA on the Agilent 2100 Bioanalyzer system?
Resolution:
Agilent offers total and small RNA assays on the Bioanalyzer...
This Information Applies To: Agilent 2100 Bioanalyzer system, 2100 Expert software
Issue:
The Agilent 2100 Bioanalyzer system does not detect a prepared microfluidic chip after it is inserted into the instrument. The interface shows the instrument...
This Information Applies To: 2100 Bioanalyzer, Bioanalyzer chips
Issue:
Can 2100 Bioanalyzer assay chips and reagents be interchanged?
Resolution:
Some assay chips can be used with multiple assays, however some cannot because the chip layout...
This Information Applies To: Agilent MassHunter GC/MS Acquisition software
Issue
The MassHunter software performs several checks during the startup sequence. If it detects a problem, an alert message box will be displayed and the message bar on...
This Information Applies To: Agilent MassHunter Quantitative Data Analysis Software
Issue:
The "NO DATA POINTS" error message appears in the Compound Information window in the lower-left corner in Quantitative Analysis. Sometimes, this problem may...
This Information Applies To: Agilent GC/MSD MassHunter with MSD ChemStation Data Analysis
Issue
When you are using the Data Analysis Report, you may need to specify a certain number of decimal places for your results different to the default decimal...