Analyze Agilent Seahorse XF Real-Time ATP rate assay data using Agilent Seahorse Analytics
Analyze Agilent Seahorse XF Hu T Cell Activation Assay data using Agilent Seahorse Analytics software
Analyze Agilent Seahorse XF Substrate Oxidation Stress…
Hello APacana ,
The reason for the difference in response between the TIC and the EIC is because when you extract the TIC of a given precursor you are extracting the ion signal from all precursors that create a 72 product ion, using your example. If…
Hello AJohnson13 ,
Here is the PC setup guide for older Windows 10 versions of MassHunter. It includes the PC specifications.
Windows10_Setup.book (agilent.com)
The software installation instructions would be included as part of your original media…
At 1 mL/min pumping water, the pressures are as follows:
Pump + Column = 53 bar
Pump + Column + 0.1 mm ID 30 cm tubing = 70 bar
Pump + Column + Tubing + Flow Cell = 75 bar
I have also confirmed that this pressure fluctuation has nothing to do…
It all depends on your following steps. You can even use Methanol or Acetonitrile for the protein precipition: Protein Precipitation for Biological Fluid Samples Using Captiva EMR-Lipid
See also the chapter on protein precipitation in Agilent's Handbook…
The Digest of upcoming webinars has been updated as of April 30.
Did you miss April's GC Live Session Recording and Discussion Thread? Check it out here: ( +) Diagnostics From Your Desk, Troubleshooting From Your Kitchen Table - Follow-up Discussion…
Hello cjwwfd ,
B.06.00 is out of support at this point, but I can offer some general suggestions for current revisions and hopefully you can apply them to your version. Version 12.0 is the current release. If possible, I would investigate your options…
I'm experimenting the same error. Logbook displays <MSQQQ_1: QqQ fault detected: 1.23 vacuum system is not ready. This is typical during a pump down cycle or when the high vacuum pump is turned off during a vent cycle or fault condition>. Does anyone…
Hello DET ,
Sorry for the late response on this.
What sort of spiking do you see usually? What is the output of the FID, in pA?
Are you using any UPS systems, or are the GCs plugged directly into the wall?
This Information Applies To: HES ion source in 5977B/C GC/MSD, 7010 triple quadrupole GC/MS systems
Issue
The filaments are replaced as part of routine maintenance, or when they break (zero output current).
Items Required
Gloves, nylon,...
This Information Applies To: Agilent 5977A/B/C GC/MSD
Issue
The Electron Multiplier Horn must be replaced when it is contaminated or the EMV value reaches 3000 V with a clean ion source.
Items Required
Electron multiplier horn (5977B/C:...
This Information Applies To: Agilent 5973/ 5975/5977 GC/MSD with rotary vane foreline pump
Issue:
The oil mist filter is recommended to be replaced annually. If the color of the filter has changed or there is smell from the filter, the oil mist...
This Information Applies To: Agilent EI Source
Issue:
During normal operation of the GC/MS, the EI SST/Inert ion source will need to be disassembled to proceed with abrasive ion source cleaning and / or routine replacement of consumable parts (filaments...
This Information Applies To: Agilent G7129A/B/C Vialsampler
Issue:
Replacing the needle and needle seat of the Vialsampler is required when:
The needle is visibly damaged.
Leaks or blockages in the vialsampler are observed, especially between...
This Information Applies To: Agilent G1311A/B/C, G7111A/B, Quaternary Pumps, and G1310A/B, G7110B Isocratic Pumps, G1312A/B/C, G7112B Binary Pumps
Issue:
How to diagnose no flow or no pressure issues on binary, quaternary, or isocratic pumps.
...
This Information Applies To: Agilent UV and RID detectors
Issue
A clogged flow cell causes high backpressure. The clog can be removed by flushing the system with solvents at high pressure. If you suspect a clog or experience high backpressure due...
This Information Applies To: Agilent G1312X and G7112X binary pumps.
Issue:
A blockage before the pressure sensor, e.g. in a pump head, will not give a high pressure or overpressure system error. Instead, the pump may show one of various error messages...
This Information Applies To: Agilent 1100, 1200, 1260 LC Pumps.
Issue:
The following procedure allows the cleaning of residue from the pump's check valves, often caused by polymerization of aged acetonitrile or salt-based solutions that may prevent...
This Information Applies To: Agilent LC Systems
Issue
After analyzing dirty or concentrated samples, some sample compounds may retain in the column and other parts of the HPLC system. If the column re-equilibration after each run is not enough to...