I had notice that the air volume is set to 1ul which is the same as the sample volume (I’m assuming is the same as the injected volume). You could try to set the air volume to 0ul and observe if the injection is consistent after this.
Hi Susana,
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Hi Mike, Thank you, yes correct, here is our situation to clarify, we upgraded in order to retire the Win7 OS. We had methods on Agilent UV 8454 ChemStation B.05.02 that managers would create, reviewers would approve, and analyst could use the methods…
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Wow, your peaks are super close to each other! Anything minor will affect your chromatogram. Maybe you should search for a better method? Anyways, what impacts the most are flow (are you running a gradient method?), organic concentration (again, gradient…
You can calculate the pressure on the flow cell by disconnecting the inlet capillary in the VWD. So the pressure in the VWD is the system pressure MINUS the pressure without VWD
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This Information Applies To: Agilent OpenLab CDS
Issue
Reports in OpenLab CDS can be printed or saved manually in Data Analysys, or automatically during reprocessing, or acquisition.
Requirements
The user needs the following Roles and Privileges...
This Information Applies To: Agilent 5975 and 5977 Series GC/MSD systems.
Issue
During tuning, MassHunter prompted the error message: "MSD Fault- RFPA Difficulty", and tune action stopped.
Steps to Follow
Try to reconfigure the GC/MS....
This Information Applies To: Agilent OpenLab CSD 2.5 and above
Issue
The integration optimizer helps find a suitable set of integration events. It allows integration parameters to be tuned via sliders (peak width, slope sensitivity, height reject...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Issue
The Agilent 2100 Bioanalyzer system run ends prematurely and the following error appears in the logbook: Run aborted on port x. Instrument error occurred on port x, Unusual...
This Information Applies To: 2100 Bioanalyzer, 2100 Expert software
Issue
The Agilent 2100 Bioanalyzer system electropherograms in the Agilent 2100 Expert software have a wavy, uneven baseline ( Figure 1 ).
Figure 1. Wavy, uneven baseline on Bioanalyzer...
This Information Applies To: Agilent AriaMx systems.
Issue
When IP addresses of an AriaMx instrument are reassigned, it will appear under the new and the previous IP address in the AriaMx Instrument Explorer. This situation can be prevented using...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
Sometimes, for support purposes, it is necessary to send the diagnostic test result file to Agilent support representatives.
This file contains detailed information about the...
This Information Applies To: Agilent AriaMx real-time PCR system, AriaMx Real-Time PCR software
Issue
How to change the discovery protocol port in the AriaMx software.
Steps to Follow
The AriaMx software uses the UDP Broadcast protocol for...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
The instrument diagnostic tests should be run whenever it is suspected that there could be a fault with the instrument hardware.
Background
The instrument diagnostics consist...
This Information Applies To: Agilent AriaMx real-time PCR system
Issue
Error 45 is displayed on the AriaMx instrument touch screen.
Background
Error 45 is shown when the temperature recorded by one or more thermistors is outside the expected...