I am building a report for some GC-FID data from a GC-MS/FID instrument. I have managed to build a report that contains the chromatogram and the table of peaks. I can scale the X-axis to the scale i need but I have been unable to scale the y-axis to show…
I am using OpenLab Chemstation C.01.10 on an 8890 with dual FIDs. I was trained on HP's version of Chemstation that reported both detector quant amounts in one row for precision purposes. It made it 10x easier to review data and confirm sample concentrations…
Varian 710 oes, nebulization is ok, fog in the chamber is omogeneous, tubing, nebulizer, torch, cone cleaned and alligned.
Work fine till 2 weeks ago, after i've experienced this: fail to execute wavelenght calibration end torch allign, No response…
Hello Everyone,
I am using 7820A GC coupled with 5977B Mass Spec. After use I close the He tank and later on I get 2 messages - 'Front inlet flow shutdown' and 'Front inlet pressure shutdown'. I restarted the software but it fails to load any GC method…
Hello, we are setting up a seahorse assay for a immunology master class. We want to measure the glycolytic rate in human PBMCs or purified human CD4 T cells activated vs non activated. We fail to see any difference. Has anyone done this kind of assay…
We have a Duo 2.5 foreline pump that was replaced in June of this year (2023). The previous Duo 2.5 installed had a sudden catastrophic leak, thus the replacement in June. The oil was filled up halfway and I set a marker with tape to monitor the level…
Hi Juliana
Yes, most likely the capacitors and/or the RF Tube. However, you can try to reduce the run power to 1.1Kw in the default ignition table. You may then be able to run for a few weeks with this reduced power level. You will need to reduce the…
I have started using GC-FID (Agilent 8890) since March for the first time. We are trying to measure the methane. I was trying to make a standard surve, so I injected 1000 ppm methane standard at first. Just to let you guys know no samples has been run…
Hi vitiin96,
this is very generic and any book on GC troubleshooting or the videos on the Agilent website could help. For the 7890 there is a troubleshooting manual available.
Good practice in GC is when you get a (new) column, you have an own suitability…
Hi byoo,
Do you get any failed to communicate or any other message when you start up the software? You are correct if the busy light should be constantly flashing. This means you got a successfuk firmware load from the PC from ethernet connection. Can…
This Information Applies To: Agilent OpenLab ChemStation Edition Software (C.01.xx)
Issue
When your Agilent OpenLab ChemStation Edition software fails to launch with errors " Login failed with the following message: Configuration system failed to...
This Information Applies To: Agilent OpenLab ChemStation Edition Software (C.01.xx)
Issue
Agilent OpenLab ChemStation Edition software fails to launch with error containing the words " Autostart Macro failed ”. This article will provide you some...
This Information Applies To: 5200/5300/5400 Fragment Analyzer, Femto Pulse, ZAG DNA Analyzer and Oligo Pro II instruments, and their respective controller software.
Issue Failed to build pressure error comes up shortly after starting a separation...
This Information Applies To: Agilent 6400 Series triple quadrupole LC/MS systems equipped with a gate valve.
Issue
Removing the Ion Transfer Capillary for maintenance on instruments with gate valve. The gate valve allows ion capillary removal and...
This Information Applies To: Agilent OpenLab ChemStation, Agilent OpenLab CDS, Agilent OpenLab EZChrom
Issue
Due to the license error “ An error occurred connecting to the license server: The desired vendor daemon is down. Check the Imgrd log file...
This Information Applies To: HPLC Advisor app
Issue
Legacy HPLC method needs to be translated to a new column or LC system (HPLC to UHPLC or Analytical to Preparative LC).
Requirements
Install the most recent version of the HPLC Advisor...
This Information Applies To: Agilent 7890 A/B, 8860 and 8890 GC Systems with Split/Splitless (SSL), Multimode (MMI) or Programmable Temperature Vaporization (PTV) Inlets
Issue:
The Inlet Leak & Restriction Test is executed after every inlet maintenance...
This Information Applies To: 5000 series ICP-OES (5100,5110,5800,5900), AVS 4/6/7, ICP Expert version 7.X
Issue
The AVS can contribute to instrument performance issues such as sensitivity and precision. During troubleshooting, bypassing (or skipping...
This Information Applies To: Agilent GC Systems
Issue
Loss of peak resolution can be a combination of decrease in separation and increased peak width. Loss of resolution can impact on analytical reproducibility and accuracy.
Other chromatographic...
This Information Applies To: Agilent GC Systems
Issue
Contamination that creates ghost and carry over peaks can be introduced at different points including sample preparation, introduction, and separation. The additional peaks can cause false positive...